高效液相色谱荧光检测法测定人血浆中多沙唑嗪的浓度  

HPLC determination of doxazosin in human plasma with fluorescence detection

作　　者：阮邹荣[1] 袁虹[1] 孙凌[1] 

机构地区：[1]浙江大学医学院附属第二医院临床药理室,杭州310009

出　　处：《药物分析杂志》2009年第2期229-232,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立人血浆中多沙唑嗪浓度的高效液相色谱-荧光检测方法。方法:以特拉唑嗪为内标。血浆样品经碱化后用乙醚萃取。采用Supelcosil ABZ+PLUS C18色谱柱(250 mm×4.6 mm,5μm),20 mmol·L-1磷酸盐缓冲液-乙腈(67∶33)为流动相,流速为1.20 mL·min-1,荧光检测波长为λex=335 nm,λem=390 nm,进样量为50μL。结果:在1.0～50.0μg·L-1浓度范围内,测定方法具良好的线性关系,线性方程为:A=0.1639C-0.0253(r=0.9998),准确度和精密度符合生物样品分析要求,最低定量浓度为1.0μg·L-1。结论:本测定方法具灵敏度高、准确和快速的优点,可用于甲磺酸多沙唑嗪人体药代动力学和生物等效性研究。Objective ：To establish a simple, sensitive and rapid RP -HPLC with fluorescence detection method for determination of doxazosin in human plasma. Methods： Terazosin was used as the internal standard. The plasma samples were extracted with ether. The solvent was evaporated to dryness at 40 ℃ in water bath. The Supelcosil ABZ ＋PLUS C18column （250 mm × 4. 6 mm,5 μm ） was used analytical column. The mobile phase consisted of 20 mmol · L^-1 potassium dihydrogenphosphate solution -acetonitrile （67：33 ） with a flow rate of 1.20 mL · min^-1 The fluorescence detector were set at λex=335 nm, λem = 390 nm. The injection volume was 50 μL. Results： A good linear relationship was obtained between the peak area ration of doxazosin/terazosin and the concentration of doxazosin over the rang of 1.0 to 50. 0 μg · L^-1. The linear equation was A =0. 1639C -0. 0253（r =0. 9998 ）. The limit of quantitative concentration was 1.0 μg·L^-1. Conclusion：The method is sensitive, accurate and simple for the determination of doazosin in human plasma. It is suitable for the pharmacokinetic and bioequivalence studies of doxazosin in human.

关 键 词：多沙唑嗪 血药浓度 高效液相色谱法 荧光检测 

分 类 号：R917[医药卫生—药物分析学]