红景天苷衍生物N03对乳鼠心肌细胞缺氧-复氧损伤的保护作用  

Protective effects of rhodioside derivative N03 on hypoxia/reoxygenation-induced injury in neonatal rat cardiomyocytes

作　　者：韩凤昭[1,2] 彭涛[1] 王林[1] 邹莉波[2] 温晓雪[1] 吕秋军[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850 [2]沈阳药科大学生命科学与生物制药学院,沈阳110086

出　　处：《中国新药杂志》2009年第3期248-252,共5页Chinese Journal of New Drugs

基　　金：国家自然科学基金项目(30472081);北京市自然科学基金(7012030)

摘　　要：目的：研究红景天苷衍生物1-C-β-D-吡喃葡萄糖基-N-（2′-氯苯甲基）甲胺（1-C-β-D-glucopyranosyl-N-（2′-chlorophenyl）methyl methanamine，N03）对乳鼠心肌细胞缺氧-复氧损伤的保护作用。方法：以1～2d龄Wistar大鼠乳鼠心室肌进行心肌细胞培养并建立缺氧-复氧损伤模型，检测不同浓度（2，10，50mg·L^-1）N03预处理后细胞的形态变化，比色法检测乳酸脱氢酶（LDH）、肌酸激酶（CK）、天冬氨酸转移酶（AST）的泄漏量及超氧化物歧化酶（SOD）活性，luminometer法检测细胞中三磷酸腺甘（ATP）水平，荧光探针负载法检测其是否能够拮抗缺氧-复氧损伤所引起的细胞内活性氧（ROS）升高和钙超载。结果：红景天苷衍生物N03能减轻心肌细胞缺氧-复氧损伤后LDH，CK和AST的漏出量，同时能够升高SOD活性，降低缺氧-复氧损伤所致的ATP水平下降程度和拮抗细胞内ROS升高和钙超载。结论：红景天苷衍生物N03对缺氧-复氧损伤的心肌细胞具有明显的保护作用，可能对心肌缺血的一些疾病具有治疗作用。Objective：To investigate the effect of rhodioside derivative N03, 1-C-β-D-glueo-pyranosyl-N- （2′-ehlorophenyl） methyl methanamine, on the injury induced by hypoxia/reoxygenation in the cultured neonatal rat eardiomyocytcs. Methods： Ventrical myocytcs from neonatal rats （1-2 days） were cultured, and pretreated with various concentration of N03 （2, 10, 50 mg·L^-1） before exposure to hypoxia/reoxygenation. Lactate dehydrogenase （LDH） in cell supernatant, ereatinc kinase （CK）, AST （aspartate transaminase）, SOD （superoxide dismutase） level, intraeellular ATP （adenosine triphosphate） , intracellular reactive oxygen species （ROS） level and Ca^2＋ were determined by colourimetry, luminometer and fluorescence probe, respectively. Results： N03 reduced LDH, CK and AST release and increased the activity of SOD after hypoxia-reoxygenation. It markedly attenuated the reduced elevation ATP and ROS, and Ca^2＋ overloading induced by hypoxia/reoxygenation. Conclusion： N03 can protect rat cardiomyocytes from the injury induced by hypoxia/rcoxygenation, suggesting a potential for the treatment of myocardial ischemia.

关 键 词：红景天衍生物 心肌缺血 缺氧-复氧 细胞保护 

分 类 号：R965.1[医药卫生—药理学] R542.2[医药卫生—药学]