生化止血饮对药物流产后大鼠子宫蜕膜细胞Fas mRNA及bcl-2 mRNA的影响  

Influence of Shenghuazhixueyin on Fas mRNA and bcl-2 mRNA of uterine decidual cell in Rats after abortion with Drugs

作　　者：于燕[1] 张燕[2] 王秀霞[1] 周景华[1] 吴效科[1] 包姗姗[2] 

机构地区：[1]黑龙江中医药大学附属第一医院,黑龙江哈尔滨150040 [2]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《现代中西医结合杂志》2009年第8期855-857,共3页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：黑龙江省自然科学基金资助项目(D200649);黑龙江省教育厅科研项目(11521264);黑龙江省哈尔滨市科技创新人才培养专项资金项目(2006RFQXS106)

摘　　要：目的探讨生化止血饮对药物流产(药流)后大鼠子宫蜕膜凋亡基因Fas mR-NA及凋亡抑制基因bcl-2 mRNA的影响及作用机制。方法建立正常对照组、模型组、益母草组、生化止血饮组,用原位杂交的方法检测凋亡基因Fas mRNA及凋亡抑制基因bcl-2mRNA的表达。结果生化止血饮可显著增加子宫蜕膜细胞凋亡基因Fasm RNA的表达,减低凋亡抑制基因bcl-2 mRNA的表达,与益母草组和模型组比较均有显著性差异(P均<0.05)。结论生化止血饮能通过增加蜕膜组织中凋亡基因Fas的表达和降低凋亡抑制基因bcl-2的表达而加速残留蜕膜从子宫壁剥脱来达到止血的目的。Objective It is to discuss the influence of Shenghuazhixueyin on apoptosis gene Fas mRNA and apoptosis inhibiting gene bcl - 2 mRNA of uterine decidua in Rats after abortion with Drugs and the mechanism of action. Methods Normal control group, model group, leonurus group and Shenghuazhixueyin group were established. The expressions of apoptosis gene Fas mRNA and apoptosis inhibiting gene bcl - 2 mRNA were detected with the method of in situ cytohybridization. Results Shenghuazhixueyin could remarkably increase the expression of apoptosis gene Fas mRNA in uterine decidual cell and reduce the expression of apoptosis inhibiting gene bcl - 2 mRNA. Compared with leonurus group and model group, there were both significant differences （P 〈 0.05）. Conclusion Shenghuazhixueyin can reach the purpose of hematischesis through increasing the expression of apoptosis gene Fas mRNA in decidual tissue and reducing the expression of apoptosis inhibiting gene bcl - 2 mRNA and then making reliquus decidua exfoliating quickly from uterine wall.

关 键 词：生化止血饮 药物流产后出血 子宫蜕膜细胞 大鼠 凋亡基因 

分 类 号：R-332[医药卫生]