铜、锌离子抗性菌筛选及重金属作用下富集特性研究  

Screen of Resistant Fungus with Copper (Ⅱ) and Zinc (Ⅱ) Ions and Study on the Characteristics of Biosorption

作　　者：刘云国[1] 周娜[1] 樊霆[1] 何义超[1] 吕志江[1] 郭帅[1] 刘来[1] 夏文斌[1] 

机构地区：[1]湖南大学环境科学与工程学院,湖南长沙410082

出　　处：《湖南大学学报（自然科学版）》2009年第2期80-84,共5页Journal of Hunan University:Natural Sciences

基　　金："十一五"国家科技支撑计划资助项目(2006BAD03A1704;2006BAD03A1706)

摘　　要：从湖南临乡桃林矿区土壤中分离到一株高抗铜、锌菌株,经26S rRNA D1/D2鉴定为Aspergillus aculeatus.该菌株单抗铜400 mg/L、锌800 mg/L.优化条件下:30℃,pH值5.0,起始离子浓度50 mg/L,摇床转速120 r/min,培养时间120 h,菌体对铜、锌离子的吸附率为54%和60%.铜、锌离子浓度50 mg/L时,菌体内谷胱甘肽含量达到最大值分别为0.98和0.88 mg/g;而正常菌体内只有0.04 mg/g.可初步认为谷胱甘肽的增加缓解了铜、锌离子对Aspergillus aculeatus的氧化损伤.A stain of resistance to copper and zinc with high concentration was isolated from soil of Taolin in Hunan Province. With the identification of 26S rRNA D1/D2 gene sequence, the strain was identified as Aspergillus aculeatus, It could resist copper of 400 mg/L and zinc of 800 mg/L. Its optimum growth conditions were at temperature of 30 ℃, pH 5.0, the initial concentration of 50 mg/L, the rate of the shaker at 120 r/min, the cultured time of 120 h, the adsorption efficiency could reach 54 % and 60 %. Under Cu2＋ , Zn2＋ at 50 mg/L, glutathione achieved the maximum, 0.98 and 0.88 mg/g, respectively, while in normal fungus it was only 0.04 mg/g. It can be assumed from the results that the increase of glutathione seems to play a favorable role in the avoidance of heavy metal oxidation stress.

关 键 词：真菌 重金属 谷胱甘肽 植物螯合肽 

分 类 号：X172[环境科学与工程—环境科学]