EGCG对结肠癌HT-29细胞基质金属蛋白酶-9表达的影响  

Effect of Epigallocatechin-3-gallate(EGCG) on MMP-9 Expression in Human Colon Cancer Cell Iine HT-29 In Vitro

作　　者：张淳[1] 周萍[1] 陈卫昌[2] 俞宪民[1] 

机构地区：[1]江苏省无锡市第三人民医院,214041 [2]苏州大学附属第一医院

出　　处：《中国微循环》2009年第1期19-21,共3页Journal of Chinese Microcirculation

基　　金：江苏省135重点医学人才基金资助(RC2007076)

摘　　要：目的探讨表没食子儿茶素没食子酸酯(EGCG)对人结肠癌细胞株HT-29基质金属蛋白酶-9(MMP-9)表达的影响。方法应用RT-PCR法和Western blot法检测EGCG干预前后HT-29结肠癌细胞中MMP-9 mRNA及MMP-9蛋白表达。结果EGCG25、50、100μg/ml作用于结肠癌细胞株HT-2948h后,MMP-9 mRNA及MMP-9蛋白的表达量随着EGCG浓度的增加而下降,结果有统计学差异(P<0.05)。结论EGCG可显著抑制HT-29细胞中MMP-9 mRNA及MMP-9蛋白的表达,呈剂量依赖性。Objective To study the effect of Epigallocatechin-3-gallate（EGCG） on matrix metalloproteinase 9（MMP-9） in human colon cancer cell line HT-29 in vitro. Methods In vitro, the MMP-9 mRNA and protein expression in human colon cancer cell line HT-29 was analyzed by RT-PCR and Western blot. Results In vitro, human colon cancer cell line HT-29 was treated by EGCG in various concentrations（ 25, 50, 100μg/ml） . After 48 hours, the expression of MMP-9 mRNA and protein in human colon cancer cell line HT- 29 were gradually decreased with the increasing dose of EGCG（ P 〈 0.05）. Conclusion EGCG could inhibit the expression of MMP-9 in HT-29 cell in vitro and in dose dependent manner, and maybe it is one of the mechanizms of the anti-tumor effect of EGCG.

关 键 词：结肠癌 表没食子儿茶素没食子酸酯 基质金属蛋白酶-9 

分 类 号：Q55[生物学—生物化学]