酪氨酸激酶抑制剂STI571对慢性粒细胞性白血病K562细胞周期的作用及机制  

Effects of Tyrosine Kinase Inhibitor STI571 on Cell Cycle of Leukemia K562 Cell Lines and Its Mechanisms

作　　者：郭晓[1] 李文静[1] 宋丹[1] 董春杰[1] 

机构地区：[1]解放军白求恩国际和平医院血液病科,石家庄050082

出　　处：《华北国防医药》2009年第1期17-19,F0002,共4页Medical Journal of Beijing Military Region

摘　　要：[摘要]目的:研究酪氨酸激酶抑制剂STI571对K562细胞周期的作用及机制。方法:以逆转录-聚合酶链反应和Western blot方法分别检测STI571处理K562细胞12、24、48 h后p38、ERK、cyclin D2、cyclin E、p27 mRNA和蛋白的表达,并以流式细胞仪检测其不同时间点细胞周期的变化。结果:STI571处理后K562细胞p38、ERK、cyclin D2、cyclin E mRNA和蛋白表达降低,p27 mRNA和蛋白表达增高。G0/G1期细胞增多,S期细胞减少,与用药前比较差异均有统计学意义(P<0·05)。结论:STI571可能通过丝裂原活化蛋白激酶途径影响细胞周期调控蛋白,最终抑制K562细胞的增殖。Objective：To study the effect of tyrosine kinase inhibitor STI571 on cell cycle of K562 cell lines and its mechanisms. Methods： The mRNA and protein expression of p38 ,ERK,cyelin D2 ,cyclin E and p27 in K562 cell lines were treated with STI571 （in 12 h,24 h,and 48 h） and detected by retrotranscription polymerase chain reaction and western blot,respectively. Cell cycle was determined by flow cytometry. Results：The mRNA and protein expressions of p38, ERK,eyclin D2 changed in different time spans and eyclin E in K562 cell lines treated with STI571 were decreased and the expressions of p27 were increased. The percentage of cells in G0/G1 phase was increased and the per- centage of cells in S phase was decreased. There was significant difference as compared with K562 cell lines before treated with STI571 ,with statistical significance（P 〈 0.05 ）. Conclusion ：STI571 can affect cell cycle regulating proteins by mitogen activated protein kinase pathway and eventually inhibit proliferation of K562 cells.

关 键 词：白血病 髓样 慢性 STI571 K562细胞 细胞周期 细胞周期蛋白类 

分 类 号：R733.72[医药卫生—肿瘤] R345.57[医药卫生—临床医学]