臭柏枝叶精油的体外抑菌活性  

In vitro Antibacterial Activity of the Essential Oil of Sabina vulgaris Branch Leaves

作　　者：郭秀艳[1] 包丽华[1] 张国盛[1] 王林和[1] 王文明 

机构地区：[1]内蒙古农业大学生态环境学院,内蒙古呼和浩特010019 [2]内蒙古乌审旗林业局,内蒙古察布达克017300

出　　处：《安徽农业科学》2009年第4期1607-1610,共4页Journal of Anhui Agricultural Sciences

基　　金：内蒙古农业大学资助项目

摘　　要：[目的]探讨臭柏精油在抑菌、抗菌领域应用前景。[方法]采用纸片扩散法、连续稀释法和自然沉降法对臭柏精油体外抗菌活性进行了测定。[结果]臭柏精油对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌、黑曲霉、酵母菌的抗菌圈直径分别为(16.2±2.3)、(8.4±1.3)、(26.2±5.1)、(16.7±2.1)、(18.0±2.4)mm;最低抑菌浓度(MIC)分别为0.50%、10.00%、0.25%、7.50%和0.50%。对室内空气中菌类的平均抑菌率达43.3%。臭柏精油对5种菌的抑菌活性强度为枯草芽孢杆菌>大肠杆菌、酵母菌>黑曲霉>金黄色葡萄球菌。[结论]臭柏精油有较强的抑菌活性,是一种很有价值和开发前景的芳香油植物资源。[ Objective ] The aim of this study was to provide the scientific basis for application of Sabina vulgaris essential oil in the antibacterial area. [ Method] Determining the antibacterial activity of the essential oil of sabina vulgaris in vitro by using disk diffusion method, serial dilution method and natural sedimentation method. [ Result ] It was found that the diameters of inhibition zone and the lowest inhibitory concentratlon （ MIC ） were ： Escherichia coli （ 16.2 ± 2.3 ） mm, 0.50 % ; Staphylococcus aureus （8.4 ± 1. 3 ） mm, 7.50% ; Bacillus subtilis （ 26.2 ± 5.1 ） mm, 0.25% ; AspergiUus niger （ 16.7 ± 2.1 ） mm, 1.00% ; Yeast （ 18.0 ± 2.4） mm ,0.50%. To the indoor bacteria, the average of inhibitory rate was 43.3%. The strength of antibacterial activity ： Bacillus subtilis 〉 Escherichia coli, Yeast 〉 Aspergillus niger 〉 Staphylococcus aureus. [ Conclusion ] Sabina vulgaris essential oil, which has quite strong antibacterial activity, enjoys a prosperous marketing prospect.

关 键 词：臭柏 精油 抑菌活性 

分 类 号：S794.9[农业科学—林木遗传育种]