杏鲍菇多糖和微量元素的含量测定  

Determination of the Content of Polysaccharide and Trace Elements in Pleurotus eryngii

作　　者：施淑琴[1] 施群[2] 

机构地区：[1]浙江省金华职业技术学院医学院,浙江金华321007 [2]浙江省金华中心医院,浙江金华321000

出　　处：《广东微量元素科学》2009年第1期58-61,共4页Trace Elements Science

摘　　要：采用苯酚-硫酸法测定了景宁产杏鲍菇中多糖的含量，并采用等离子体发射光谱法（ICP—AES法）测定了其微量元素的含量。结果表明，多糖测定波长为490nm，在2．04～10．20μg／mL范围内吸光度与被测物含量呈良好的线性关系，r=0．9992，方法的平均回收率为100．8％，RSD=1．3％。杏鲍菇含有丰富的多糖及铜、铁、锰、锌、硒等微量元素，药食两用，具有很高的经济价值和药用价值。多糖测定方法简便、准确、重现性好，可作为杏鲍菇多糖含量的测定方法。The content of polysaccharide and trace elements was determined by phenol - sulfuric acid method and ICP Pleurotus erynglii produced in Jingning AES. The tetermination wavelength of polysaccharide was 490 nm. The good linearity rang from 2.04 - 10. 20 μg/mL, r =0. 999 2, recovery of the method was 100. 8%, RSD = 1.3%. The results show there is higher content of polysaceharide and copper, iron, manganese, zinc and selenium, edible drugs, medicinal value. The method is simple and accurate. It can be used have high economic value and for determination of polysaccharide in Pleurotus erynglii.

关 键 词：杏鲍菇 多糖 微量元素 苯酚-硫酸法 等离子体发射光谱法 

分 类 号：O657.32[理学—分析化学]