“活性甲醛”与甲醛远距离毒性的初步研究  

Preliminary study on the activity of formaldehyde and its distant-site toxicity

作　　者：娄小华[1] 陈莉[1] 吴丹[1] 付小沫[1] 李学彬[1] 赵伟[1] 曹毅[1] 杨旭[1] 

机构地区：[1]华中师范大学生命科学学院环境科学实验室,武汉430079

出　　处：《环境科学学报》2009年第3期607-612,共6页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金面上项目(No.30570799)~~

摘　　要：甲醛是否具备远距离毒性(distant-sitetoxicity)是揭示甲醛与白血病关系的关键问题.本研究采用DPC、MTT实验方法检测甲醛的远距离毒性,结果发现当把等剂量的甲醛与GSH联合作用对细胞进行染毒后细胞内产生的DPC系数比单独同剂量的甲醛组要高很多,在MTT实验中发现在单独甲醛组中加入等量的GSH后细胞活性也出现了显著下降;在动物实验中,当对小鼠肝染毒后脑组织中也同样出现了损伤.说明甲醛在机体内与GSH形成了结合物,这种结合物可能协助甲醛完成跨膜并进入到局部组织再次释放出游离态甲醛,实现了甲醛的远距离毒性,这种结合态的甲醛称之为"活性甲醛".The distant-site toxicity of formaldehyde is a key question concerning the relationship of formaldehyde and leukemia. In this study we used DNA-Protein Crosslinks （DPC） and MTT assays to detect the distant-site toxicity of formaldehyde. The results showed that the DPC coefficient of the combined group was much higher than that of the separate group exposed to the same dose of formaldehyde, however, we found the cell activity dropped significantly in the combined group compared to the separate group. The same result was also found in animal experiments. When mouse liver was exposed to formaldehyde, the brain tissue was damaged. In the body, formaldehyde can form a complex with GSH, which may help the formaldehyde cross the cell membrane and re-enter formaldehyde-free cells, inducing a distant-site toxicity. The formaldehyde-GSH complex may be regarded as ＂ active formaldehyde＂.

关 键 词：甲醛(FA) 谷胱甘肽(GSH) DNA-蛋白质交联(DPC) 细胞活性检测 远距离毒性 

分 类 号：X171.5[环境科学与工程—环境科学]