Effect of RNA Interference Hsp72 Gene Expression on Development of Mouse Preimplantation Embryos  

作　　者：WANG Ming-zhi TIAN Wen-ru TIAN Zhong-jie QU Ping-ping GAO Shan-song ZHANG Zhi-hong ZHANG Qi-yao HOU Chun-xia LI Hui-hui 

机构地区：[1]College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, P.R.China

出　　处：《Agricultural Sciences in China》2009年第2期223-230,共8页中国农业科学（英文版）

基　　金：the National Natural Science Foun-dation of China (30571338) for financial support.

摘　　要：The method of RNAi was used to inhibit the expression of induced heat shock protein 70 （Hsp72） in the 4-cell stage mouse embryos and the embryo development competence was analyzed to identify the functions of Hsp72 on embryonic heat resistance. The results indicated that the inhibition rates of siRNA1 for Hsp72 mRNA and Hsp72 protein were 87.1 and 78.5%, respectively. The blastocysts development rates were 41, 86, and 84% for the siRNA1 group, the LipofectamineTM 2 000 exposed group, and the 37℃ group, respectively, and the hatched blastocysts development rates for the above three groups were 35, 72, and 68%, respectively. The data suggest that the siRNAI has a significant inhibiting effect on Hsp72 gene, and Hsp72 gene silence reduces the blastocysts development rate and hatched blastocysts rate after heat shock during the development of mouse preimplantation embryos.The method of RNAi was used to inhibit the expression of induced heat shock protein 70 （Hsp72） in the 4-cell stage mouse embryos and the embryo development competence was analyzed to identify the functions of Hsp72 on embryonic heat resistance. The results indicated that the inhibition rates of siRNA1 for Hsp72 mRNA and Hsp72 protein were 87.1 and 78.5%, respectively. The blastocysts development rates were 41, 86, and 84% for the siRNA1 group, the LipofectamineTM 2 000 exposed group, and the 37℃ group, respectively, and the hatched blastocysts development rates for the above three groups were 35, 72, and 68%, respectively. The data suggest that the siRNAI has a significant inhibiting effect on Hsp72 gene, and Hsp72 gene silence reduces the blastocysts development rate and hatched blastocysts rate after heat shock during the development of mouse preimplantation embryos.

关 键 词：RNA interference MOUSE BLASTOCYST Hsp72 gene 

分 类 号：Q954.4[生物学—动物学] Q78