一株嗜热纤维素分解菌的分离及其酶学性质的初探  

Initial Studies on Isolation of a Thermophilic Cellulose-degrading Bacteria and its Enzymology Characteristics

作　　者：曲小爽[1,2] 顿宝庆[1] 郭明鸣[1,2] 宋立立[1,3] 许修宏[2] 张保明[1] 路明[1] 李桂英[1] 

机构地区：[1]中国农业科学院作物科学研究所,国家农作物基因资源与基因改良国家重大科学工程,中国农业科学院生物质能源研究中心,北京100081 [2]东北农业大学生命科学学院,哈尔滨150030 [3]西北农林科技大学,陕西杨凌712100

出　　处：《中国农业科技导报》2009年第1期124-128,共5页Journal of Agricultural Science and Technology

基　　金：“十一五”国家科技支撑计划项目(2006BAD07A04);农业部农村能源综合建设项目(2130138-0-08);中央级公益性科研院所基本科研业务费专项资金(2060302-13)资助

摘　　要：利用海南热泉附近腐烂朽木及土壤,筛选分离到一株具有较高纤维素酶活性的纤维素分解菌WQ-50,经生理生化特性及遗传分析初步鉴定其为环状芽孢杆菌属菌株。当以复筛培养基发酵培养时,该菌株在55℃、pH 6条件下生长较好。在该条件下,以经碱预处理的玉米秸秆粉为唯一碳源发酵培养5 d时产酶达到最大值,羧甲基纤维素酶活性为6.8 IU/mL,滤纸酶活性为3.5 IU/mL。WQ-50 was isolated from decayed wood and soil near hot spring in Hainan. It was identified as Bacilluscirculans by preliminary physiological and biochemical characteristics and genetic analysis. When fermented by CMC-Na culture medium, strain WQ-50 strain could grow well under PH 6 and 55℃. Under this condition, when fermented by zymolytic culture medium with alkalized pretreated corn stalk powder as the sole carbon source, enzyme production reached maximum after 5 days, the CMCase activity of WQ-50 is 6.8 IU/mL and FPA activiy of WQ-50 is 3.5 IU/mL.

关 键 词：嗜热纤维素分解菌 筛选 纤维素酶 鉴定 

分 类 号：S216.2[农业科学—农业机械化工程]