水稻光敏与温敏核不育基因之间互作效应与利用研究  

Utilization of Recombination Effect of PGMS Genes and TGMS Genes of Rice

作　　者：李新奇[1] 袁隆平[1] 邓华凤[1] 欧爱辉[1] 邓启云[1] 段美娟[1] 李雅礼[1] 

机构地区：[1]国家杂交水稻工程技术研究中心,长沙410125

出　　处：《科技导报》2009年第3期74-79,共6页Science & Technology Review

基　　金：中国高技术研究发展计划(863计划)项目(2006AA10Z1F3)

摘　　要：选育不育期败育彻底、不育性稳定的光、温敏不育系是目前两系法杂交稻制种安全可靠的重要保证。本研究利用光敏不育系7001S与安全型温敏不育系广博S,广培S杂交,将农垦58S光敏不育基因与安农S温敏不育基因重组在一起,进行了重组体的不育性和不育稳定性研究。安农S核不育基因使得重组型光敏不育系的不育性变得非常稳定,不同来源的重组型光敏不育系互交杂种F2代光敏不育株率达70%以上,容易从中筛选到不育起点温度低的重组型光敏不育系。将光敏不育基因回交转移到广博S和广培S中,得到与广博S或广培S相似的重组型光敏不育系光广博S,光广培S,其不育起点温度提高。用光广博S(或光广培S)为父本与广博S(或广培S)杂交产生了不育起点温度极低的温敏不育F1代;用广博S与其重组型温敏不育近等基因系光温广博S杂交,其F1代不育起点温度也很低。研究表明,光敏不育基因与温敏不育基因重组,能够解决光敏不育系难获得和败育不彻底的问题;短日高温下,光敏不育性对温敏不育性表现上位作用;控制农垦58S不育类型不育起点温度表达的遗传因子与安农S不育类型的很可能不同,建立安全型温敏不育系的带有光敏不育基因的近等基因系(重组型光敏不育系或者重组型光温敏不育系),用其作为父本与安全型温敏不育系杂交,其F1代可代替安全型温敏不育系用于生产。By recombining the photo-sensitive Genic Male Sterile （PGMS） gene from Nongken 58S and the thermo-sensitive Genie Male Sterile （TGMS） genes from AnnongS, it was found that the frequency of PGMS plants was greatly increased, and abundant PGMS plants with various characteristics were obtained in the filial generations, and the male sterility of the rPGMS （recombinogenic PGMS and TGMS genes） plants was complete and stable. The recombination of PGMS and TGMS genes can not only solve the problems of incomplete sterility of PGMS lines, but also provide a very effective way to develop various types of elite rPGMS lines. By crossing an rPGMS line with another rPGMS line, more than 70% of F2 plants were found to be photo-sensitive male sterile. Based on the method, several rPGMS lines with low critical sterility-inducing temperature were developed in our study. When an sTGMS line （TGMS line with lowest critical sterility-inducing temperature） was crossed with its near iso-genic line, an rPGMS line, an rPTGMS line （TGMS line with PGMS and TGMS genes） or a TGMS line with high critical sterility-inducing temperature, the Fls saw a lowest critical sterility-inducing temperature and can be used to replace sTGMS lines in hybrid seed production, sTGMS lines were also muhiplied by planting in a green house with supplementary pollination.

关 键 词：重组型光敏不育系 安全型温敏不育系 不育基因 繁殖 

分 类 号：S511[农业科学—作物学]