空间搭载高产葡萄糖耐量因子(GTF)酵母的选育  

Selection of a High Yield Yeast Strain of Glucose Tolerance Factor After Space Piggyback Experiment

作　　者：刘鹭[1] 吕加平[1] 高艳红[2] 

机构地区：[1]中国农业科学院农产品加工所,北京100193 [2]内蒙古农业大学食品科学与工程学院,内蒙古呼和浩特010018

出　　处：《微生物学通报》2009年第2期223-230,共8页Microbiology China

基　　金：国家航天育种工程(No.2006HT00013)

摘　　要：以从本试验室收藏的7株酵母菌种及其7株经空间搭载(实践八号)诱变后的酵母菌株为研究对象,通过梯度含铬YEPD平板培养和液体YPD培养基发酵试验,利用氨水提取菌体中的GTF和火焰原子吸收光谱法检测其有机铬含量,从中筛选出一株葡萄糖耐量因子的高产酵母(YS-3),有机铬含量达1296μg/g,总铬含量达1926μg/g,生物量为40g/L。同时分析了菌体发酵过程中,菌体有机铬富集量与其它发酵参数的动态关系。A strain of high-biomass, producing glucose tolerance factor yeast strain was screened from seven yeast strains and seven strains mutated by spaceflight-induced mutagenesis through gradient concentration of chromium plates cultivation and liquid fermentation cultivation. The biomass of it was 40 g/L. Extracting GTF with ammonia and determining the content of Cr with flame atomic absorption spectrometry, the content of organic Cr was 1287 μg/g and that of total Cr was 1917 μg/g. At the same time, dynamic relationship between other parameters and the amount of organic chromium was analyzed during fermentation.

关 键 词：空间搭载 葡萄糖耐量因子 GTF 有机铬 

分 类 号：TQ926[轻工技术与工程—发酵工程]