序贯试验设计改进高效硝化菌发酵培养基  

Improvement of Fermentation Medium for High-Efficiency Nitrobacteria by Sequential Experimental Design

作　　者：任杰[1] 林炜铁[1] 罗晓春[1] 谢明权[1] 

机构地区：[1]华南理工大学生物科学与工程学院,广东广州510006

出　　处：《华南理工大学学报（自然科学版）》2008年第12期91-96,共6页Journal of South China University of Technology(Natural Science Edition)

摘　　要：为了开发商品化的硝化菌产品,以去除养殖水体中的亚硝酸盐,利用序贯试验设计(包括Plackett-Burman设计、部分析因设计、最速上升法和中心组合设计等)对从虾塘中筛选得到的硝化菌的发酵培养基进行优化,得到最佳培养基组成为:NaHCO31.86g/L、NaNO22.04g/L、Na2CO30.2g/L、NaCl 0.2 g/L、KH2PO40.1 g/L、MgSO4.7H2O 0.1 g/L和FeSO4.7H2O0.01g/L.由该优化培养基培养的硝化菌使亚硝酸盐的氧化速率由初始的580.7mg/(g.d)提高至859.5mg/(g.d),且其在模拟和真实养殖水体中对亚硝酸盐的降解效果良好.In order to produce commercial nitrobacteria for the removal of nitrites in aquaculture water, the sequential experimental design including the Plackett-Burman design, the factorial design, the steepest ascent method and the central composite design was used to optimize the fermentation medium of nitrobacteria isolated from a shrimp pond. The optimal composition of the medium was determined as 1.86 g/L NaHCO3, 2.04 g/L NaNO2, 0. 2 g/L Na2CO3, 0. 2g/L NaCl, 0. 1 g/L KH2PO4, 0. 1 g/L MgSO4 ·7H20, and 0. 01 g/L FeSO4 ·7H2O. The results indicate that, by using the nitrobacteria cultured with the optimal medium, the nitrite oxidation rate increases from 580. 7mg/（g · d） to 859.5 mg/（ g · d）, and the nitrites in both the simulated and the field aquaculture systems can effectively be oxidized.

关 键 词：硝化菌 培养基 优化 序贯试验设计 PLACKETT-BURMAN设计 最速上升法 中心组合设计 

分 类 号：Q819[生物学—生物工程]