纳米活性炭吸附氟尿嘧啶在胃癌荷瘤裸鼠体内释放及抑瘤作用的观察  

Sustained release and antitumor effectiveness of fluorouracil bound to activated carbon nanoparticles in xenotransplantation nude mice

作　　者：董怡民[1] 沓小容[2] 姚崇舜[2] 徐惠绵[1] 

机构地区：[1]中国医科大学附属第一医院肿瘤外科,辽宁沈阳110001 [2]沈阳药科大学药剂科,辽宁沈阳110001

出　　处：《中华肿瘤防治杂志》2008年第23期1768-1770,共3页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金(30672050)

摘　　要：目的:观察自制的5-氟尿嘧啶(5-FU)-纳米活性炭(ACNP)在胃癌荷瘤裸鼠体内的释放和抑瘤效果。方法:在BALB/c/nu裸鼠腹腔内移植人胃癌细胞系SGC7901,建立转移模型并随机分组。A组:腹腔内注入5-FU;B组:5-FU-AC-NP;C组:纯生理盐水。1)于不同时间点内眦静脉采血,高效液相色谱检测血浆中5-FU浓度;2)观察各组小鼠的存活状况,比较各组腹腔内瘤结节的质量和大小。结果:5-FU-ACNP组与5-FU组比较,前者血中的5-FU吸收、消除半衰期延长,峰浓度减低且变化平稳,P<0.05;裸鼠腹腔内瘤结节较轻、较少,P<0.05,裸鼠的中位存活时间延长,P≤0.01。结论:自制的ACNP结合5-FU经腹腔给药后,药物入血缓慢,血浆中的浓度低,半衰期延长,毒副反应减轻,对裸鼠腹腔移植瘤有较强抑制作用。OBJECTIVE：To observe the releasing and antitumor effectiveness of fluorouracil bound to activated carbon nanoparticles in nude mice.METHODS：Human gastric carcinoma cell strain SGC7901 was transplanted into the abdominal cavities of BALB/c/nu mice to make a xenotransplantation model and three groups were divided randomly.5-FU,5-FU-ACNP and physiological saline solutions were intraperitoneally injected respectively.The blood samples were collected at different time points and the 5-FU concentration,tumors＇ weight and the mice survival time were measured in all the groups.RESULTS：Compared with the 5-FU group,the absorption phase and the elimination half-life were extended（P〈0.05）,the peak concentration of 5-FU in serum was degraded and stable,the weight of transplanted tumors was light（P〈0.05）,the median survival time extended significantly in the 5-FU-ACNP group（P≤0.01）.CONCLUSIONS：After intraperitoneal administration of 5-FU-ACNP,5-FU is taken into blood slowly and the concentration level in the serum is low and stable,and the poisonous effect is weakened.In the abdominal cavity of nude mice,the transplanted tumor can be inhibited by 5-FU-ACNP.

关 键 词：氟尿嘧啶/药理学 胃肿瘤/病理学 炭 吸附 纳米技术 小鼠 裸 

分 类 号：R735.2[医药卫生—肿瘤]