豆豉对早期动脉粥样硬化大鼠主动脉平滑肌细胞凋亡的影响  

Effects of Semen Sojae Preparatum on the Apoptosis of Aortic Smooth Muscle Cells in Rats With Early Atherosclerosis

作　　者：牛丽颖[1] 田鹏娜[2] 李清[1] 刘娇[1] 王鑫国[1] 

机构地区：[1]河北医科大学中医学院,河北石家庄050091 [2]保定市第三医院药剂科,河北保定071000

出　　处：《大豆科学》2009年第1期156-159,共4页Soybean Science

摘　　要：观察豆豉对早期动脉粥样硬化大鼠主动脉平滑肌细胞凋亡及相关凋亡基因突变型p53和Fas蛋白表达的调节作用,以探讨其机制。采用高脂饲料喂养法建立大鼠早期动脉粥样硬化模型,同时连续10周灌胃给予豆豉提取物进行干预。末次给药后处死动物,光镜下观察血管平滑肌细胞的形态,透射电镜观察超微结构,采用流式细胞术检测细胞凋亡率以及凋亡相关基因突变型p53和Fas蛋白的表达。模型对照组主动脉平滑肌细胞的凋亡率及增值指数(FI)明显高于正常对照组(P<0.05),p53蛋白表达上调;豆豉干预组(20 g生药.kg-1、40 g生药.kg-1)大鼠主动脉平滑肌细胞的凋亡率明显高于模型对照组(P<0.05),增殖指数明显低于模型对照组(P<0.05),Fas蛋白表达上调(P<0.05),p53蛋白表达下调(P<0.05),光镜及透射电镜可见主动脉损伤明显较模型对照组改善。豆豉可调节早期动脉粥样硬化大鼠血管平滑肌细胞凋亡与增殖的平衡,其机制可能与调节突变型p53和Fas蛋白的表达有关。To investigate the effects of Semen Sojae Preparatum（SSP）on apoptosis, and gene expression of p53 and Fas in aortic smooth muscle cells （ASMCs）in early Atherosclerosis （AS）rats, and to explore the possible mechanism. Rat models with early AS were induced by feeding fatty foods, meanwhile rats in two treated groups were orally administered with extract of SSP for 10 weeks. All rats were killed and samples of ASMCs harvested. The cellular morphology was observed by light microscope and ultrastructure by transmission electron mieroseopy;apoptosis and gene expression of p53 and Fas were detected by flow eytometry. Compared with normal group, the apoptosis rate and the proliferation index （PI） in model group were obviously increased（ P 〈 0. 01 ） ,with higher gene expression of 1）53 ;moreover compared with model group ,the apoptosis rates in two treated groups （20 g · kg^-1,40 g · kg^-1 ） were higher （ P 〈 0.01 ）, the proliferation index was lower （ P 〈 0. 01 ）, and the gene expression of Fas was increased, but p53 was decreased（ P 〈 0.05 ） ;histologically, the aortic damage was remarkably improved with SSP. These results suggest that SSP can adjust the balance between apoptosis and proliferation of ASMC ,which may relate to gene expression regulation of p53 and Fas.

关 键 词：豆豉 动脉粥样硬化 细胞凋亡 增殖指数 突变型P53 Fas 

分 类 号：R285.5[医药卫生—中药学]