肝纤维化肝硬化患者肝组织中IGFBP-2,6,7的表达及意义(英文)  

Expression of IGFBP-2,-6,and-7 in patients with hepatic fibrosis and cirrhosis and its significance

作　　者：刘立新[1,2,3] 黄帅[1] 张海燕[1,3] 田小霞[2] 

机构地区：[1]山西医科大学第一临床医学院科研实验中心,太原030001 [2]山西医科大学肝病研究所 [3]山西省部共建教育部细胞生理学重点实验室

出　　处：《山西医科大学学报》2009年第1期1-5,共5页Journal of Shanxi Medical University

基　　金：国家自然科学基金资助项目(30740031);国家教育部新世纪优秀人才支持计划资助项目(NCET-06-0264)

摘　　要：目的研究胰岛素样生长因子结合蛋白(IGFBP)在肝纤维化和肝硬化患者肝组织中的表达、分布和意义。方法对18例肝纤维化、18例肝硬化患者肝组织和6例对照组(为手术切除的肝血管瘤外的正常肝组织)标本分别进行HE染色、VG染色,以及TGF-β1、TGFRΙ、胶原纤维、IGFBP-2,-6,-7及CD14的免疫组织化学染色,并将其通过图像分析系统进行半定量分析和相关分析。结果对照组正常肝组织IGFBP-2,-6,-7的表达表现为极少数肝细胞、中央静脉和胆管上皮细胞有阳性染色,在肝纤维化组和肝硬化组的表达主要见于肝窦内和纤维间隔。并且IGFBP-2,-6,-7在对照组正常肝组织、肝纤维化组和肝硬化组肝组织的表达逐渐增高,组间差异有统计学意义(P<0.05)。定量分析肝组织IGFBP-2,-6,-7的表达分别与肝组织胶原纤维、TGF-β1、TGFRΙ、CD14的表达呈正相关。结论IGFBP-2,-6,-7在人肝纤维化的发生发展中可能起重要作用。Objective To investigate the expression and distribution of IGFBP-2,-6, and -7 in different stages of hepatic fibrosis and e- ventually cirrhosis in vivo. Methods Forty-two liver specimens were collected from 18 cases of hepatic fibrosis （fibrosis group）, 18 cases of hepatic cirrhosis （ cirrhosis group）, and 6 cases of normal hepatic tissues after partial hepatectomy of hepatic hemangioma （ control group）and observed by HE staining and VG staining. Expression of TGF-β1 ,TGFRI,collagen fiber（ Cf）, IGFBP-2,-6, and -7 and CD14 was detected and semiquantitatively analyzed by immunohistochemistry staining. Results In control group immunohistochemistry revealed that just a small mount of positive staining of IGFBP-2,-6, and -7 was detected upon a few hepatocytes, central veins, and tube wall of bile duct. However, compared with control group, the expression of IGFBP-2, -6, and -7 significantly up-regulated in hepatic sinusoid and around fibrous septa in fibrosis and cirrhosis groups （ P 〈 0.05 ）. Furthermore, the expression of IGFBP-2, -6, and -7 was higher in cirrhosis group than in fibrosis group. The high expression of IGFBP-2, -6, and -7 in hepatic tissues was correlated positively with TGF-131 ,TGFRI, CD14, and collagen fiber（Cf）. Conclusion IGFBP-2, -6, and -7 may play an important role in the occurrence and development of hepatic fibrosis and cirrhosis.

关 键 词：肝纤维化 肝硬化 胰岛素样生长因子结合蛋白 

分 类 号：R575[医药卫生—消化系统]