H2O2诱导ECV304细胞凋亡与Cyclophilin—D蛋白表达的研究  

Apoptosis of the endothelial cells induced by H202 and expression of Cyclophilin-D protein

作　　者：彭江洲[1] 陈炬[1] 华平[1] 陈柏深[1] 薛雷[1] 杨艳旗[1] 

机构地区：[1]中山大学附属第二医院心胸外科,广州510120

出　　处：《中华实验外科杂志》2009年第3期333-335,F0003,共4页Chinese Journal of Experimental Surgery

基　　金：基金项目：国家“985”基金资助项目（81000-3253382）

摘　　要：目的探讨人脐静脉内皮细胞凋亡模型中Cyclophilin—D蛋白的表达及意义。方法分别应用100、300、500μmol／LH2O2按时间依从处理人脐静脉ECV304细胞，对照组单纯磷酸盐缓冲液（PBS）处理。检测细胞凋亡率、Cyclophilin—D蛋白及其编码基因ppif的表达量的变化。结果500μmol／LH2O2处理组的ECV304细胞凋亡率、Cyclophilin—D蛋白表达量和Cyclophilin—D编码基因ppif的表达量均高于对照组（P〈0．01）。Cyclophilin—D表达量与H2O2作用时间和凋亡率呈显著正相关，r分别为0．967（P〈0．01）和0．971（P〈0．01）。结论H2O2可成功诱导血管内皮细胞氧化损伤与凋亡，Cyclophilin—D蛋白可作为细胞凋亡严重程度的预测因子。Objective To investigate the significance of cyclophi]in-D protein expression in the apoptotie endothelial ceils. Methods The apoptois model of ECV304 ceils was established by 100,300 and 500 μmol/L H2O2. The control group was treated by PBS. Parameters observed included apoptosis rate,the level of cyelophilin-D and ppif gone expression. Data were analyzed by SPSS13. Results Compared with the control group, apoptosis rate of ECV304 cells,the expression of cyclophilin-D increased,and the expression of ppif gene were increased （ P 〈 0.01 ） in the 500 μmoL/L H2O2 group. The level of cyclophilin-D expression was positively correlated with the effect time of H2O2 （ r =0.967 ,P 〈0.01 ） and apoptosis rate （r = 0.971, P 〈 0.01 ）. Conclusion H2O2 may successfully induce oxidative injury and apoptosis of the endothelial cells in human blood vessels. The expression of eyclophilin-D protein may prodiet the apoptosis of the endothelial cells.

关 键 词：内皮细胞 脱噬作用 CYCLOPHILIN D蛋白 

分 类 号：R285[医药卫生—中药学]