Expression of 6 MicroRNAs in Prostate Cancer and Its Significance  

作　　者：Ming Li Liyu Cao Hongfu Zhang Yu Yin Xiaochun Xu 

机构地区：[1]Department of Pathology, Anhui Medical Uni versity, Hefei 230032, Anhui Province, China [2]Department of Clinical Cancer Prevention, UT M. D. Anderson Cancer Center, Houston, Texas. 77072 USA

出　　处：《Chinese Journal of Clinical Oncology》2009年第1期21-28,共8页中国肿瘤临床（英文版）

摘　　要：OBJECTIVE Numerous microRNAs (miRNAs) are deregulatedin human cancers. The experimental evidence supports thatmiRNAs plays a role in the initiation and progression of humanmalignancies.The present study was undertaken to evaluatethe differential expression of 6 miRNAs as biomarker for earlydetection of prostate cancer, and then to determine whether theexpression profiling of these miRNAs could predict the prognosisof prostate cancer.METHODS The expression profilings of these 6 miRNAs wereinvestigated using the method of locked nucleic acid (LNA)-modified oligonucleotide in situ hybridization (ISH). And thetechnology of tissue microarray (TMA) was employed using theformalin-fixed, paraffin-embedd (FFPE) specimens taken from52 patients with prostate carcinoma (PCa) and 38 patients withbenign prostatic hyperplasia (BPH).RESULTS The rates of positive expression for 6 miRNAs (miR-15b, miR-16, let-7g, miR- 96,miR-182 and miR-183) were 26.92%,15.38%, 15.38%, 67.31%, 61.54% and 71.15% in the specimens ofprostate cancer, and 57.89%, 76.32%, 68.42%, 44.74%, 31.58%,47.37% in the tissues of benign prostatic hyperplasia, respectively.The expressions of all 6 miRNAs between the prostate cancer andbenign prostatic hyperplasia tissues were significantly different(P < 0.05). The positive rate of these 6 miRNAs was significantlyrelated to the Gleason Grading of prostate cancer (P < 0.01). Therewas no significant correlation between the expression of thesemiRNAs and age and the concentration of serum PSA of thepatient (P >0.05). We also found that the expression of miR-15b,miR-96 and miR-182 correlated with clinical stages of tumor (P <0.05). The expression of miR-96 correlated with lobus prostatae oftumor invasion (P < 0.01), but the expressions of the remaining fivemiRNAs were not correlated with that (P >0.05). In addition, theexpression of miR-15b was negatively related to that of miR-96,miR-182 and miR-183, respectively (P < 0.01, r < 0.00).There wasa positive correlation among the expressions of miR-96, miR-182and miR-183 in OBJECTIVE Numerous microRNAs （miRNAs） are deregulated in human cancers. The experimental evidence supports that miRNAs plays a role in the initiation and progression of human malignancies.The present study was undertaken to evaluate the differential expression of 6 miRNAs as biomarker for early detection of prostate cancer, and then to determine whether the expression profiling of these miRNAs could predict the prognosis of prostate cancer. METHODS The expression profilings of these 6 miRNAs were investigated using the method of locked nucleic acid （LNA）- modified oligonucleotide in situ hybridization （ISH）. And the technology of tissue microarray （TMA） was employed using the formalin-fixed, paraffin-embedd （FFPE） specimens taken from 52 patients with prostate carcinoma （PCa） and 38 patients with benign prostatic hyperplasia （BPH）. RESULTS The rates of positive expression for 6 miRNAs （miR- 15b, miR-16, let-7g, miR- 96,miR-182 and miR-183） were 26.92%, 15.38%, 15.38%, 67.31%, 61.54% and 71.15% in the specimens of prostate cancer, and 57.89%, 76.32%, 68.42%, 44.74%, 31.58%, 47.37% in the tissues of benign prostatic hyperplasia, respectively. The expressions of all 6 miRNAs between the prostate cancer and benign prostatic hyperplasia tissues were significantly different （P 〈 0.05）. The positive rate of these 6 miRNAs was significantly related to the Gleason Grading of prostate cancer （P 〈 0.01）. There was no significant correlation between the expression of these miRNAs and age and the concentration of serum PSA of the patient （P 〉 0.05）. We also found that the expression of miR-15b, miR-96 and miR-182 correlated with clinical stages of tumor （P 〈 0.05）. The expression of miR-96 correlated with lobus prostatae of tumor invasion （P 〈 0.01）, but the expressions of the remaining five miRNAs were not correlated with that （P 〉 0.05）. In addition, the expression of miR-15b was negatively related to that of miR-96, miR-182 and miR-183, respectively （P �

关 键 词：MICRORNA prostate cancer benign prostatic hyperplasia tissue microarray in situ hybridization. 

分 类 号：R394.1[医药卫生—医学遗传学] R737.25[医药卫生—基础医学]