反转录-聚合酶链式反应检测促血管生成素-1和促血管生成素-2及Tie-2受体的mRNA在血管瘤组织中的表达及其意义  

Expression of Ang-1, Ang-2 and Tie-2 receptor mRNAs and their implications in hemangioma by RT-PCR

作　　者：洪帆[1] 庄福连[1] 王彪[1] 林经安[2] 

机构地区：[1]福建医科大学附属第一医院整形外科,福州350005 [2]福建医科大学附属第一医院中心实验室,福州350005

出　　处：《中华医学美学美容杂志》2009年第1期49-52,共4页Chinese Journal of Medical Aesthetics and Cosmetology

基　　金：福建省教育厅科研基金资助项目（编号：JA05266）

摘　　要：目的研究血管瘤组织中促血管生成素-1（Ang-1）、促血管生成素-2（Ang-2）及Tie-2受体的mRNA表达水平，探讨其在血管瘤血管生成中的作用与意义。方法用反转录-聚合酶链式反应（RT—PCR）的方法分别检测30例血管瘤组织（增生期血管瘤17例，消退期血管瘤13例）以及10例小儿正常皮肤组织中Ang-1mRNA、Ang-2mRNA及Tie-2mRNA的表达水平。用免疫组织化学方法检测血管瘤组织Ki-67蛋白的表达。结果增生期血管瘤组织中Ang-2mRNA及Tie-2mRNA表达水平高于消退期血管瘤组织（P〈0．01）。血管瘤组织中Ang-1mRNA表达为阴性。结论Ang—Tie-2体系表达失衡在血管瘤的发生发展过程中可能起重要的作用。Objective To detect the expression level of Ang-1, Ang -2 and Tie-2 receptor mRNAs in the hemangioma tissuses and to explore the effects of Ang-1, Ang-2, and Tie-2 receptor on the anglo- genesis in the hemangioma. Methods By use of serniquantinative reverse transcription polymerase chain reaction, we detected the expression level of Ang-1 mRNA, Ang-2 mRNA and Tie-2 mRNA in 30 cases of hemangioma （17 specimens of proliferating hemangioma, and 13 involuting hemangioma ）together with 10 cases of normal skin as control, and the expression level of Ki-67 protein was also detected by an immunohistochemiea method. Results The expression of Ang-2 mRNA and Tie-2 mRNA in the proliferating hemangiomas was higher than that in involuting hemangiomas （P〈0.01） . The expression of Ang-1 mRNA in hemangiomas was negative. Conclusion The unbalance of Ang-Tie-2 system is probably important for the pathogenesis and development in hemangiomas.

关 键 词：血管瘤 促血管生成素 TIE-2受体 细胞增殖相关蛋白Ki-67 

分 类 号：R732.2[医药卫生—肿瘤]