人卵巢癌相关抗原OVA66的B细胞表位预测  

Prediction of B-cell epitopes on human ovarian cancer-associated antigen OVA66

作　　者：刘争春[1] 王颖[1] 王树军[1] 张惠珍[1] 荣婷婷[1] 王庆飞[1] 谢国化[1] 葛海良[1] 

机构地区：[1]上海交通大学医学院,上海市免疫学研究所,200025

出　　处：《实用医学杂志》2009年第4期513-516,共4页The Journal of Practical Medicine

基　　金：上海市教育委员会科研创新项目(编号:08YZ39);上海交通大学医学院博士点建设基金项目(编号:DXJ0705)

摘　　要：目的:预测人卵巢癌相关抗原OVA66的B细胞表位。方法:B细胞表位预测,以单参数(亲水性、可及性、柔韧性、极性)预测为基础,结合ABCpred预测结果,并经二级结构预测筛选等综合分析。结果:OVA66的B细胞表位可能位于209-216,266-275,294-301,362-368和391-396氨基酸残基的区域内或附近。结论:该结果对应用合成肽抗原进行肿瘤患者的早期诊断研究具有重要指导意义。Objective To predict B-cell epitopes on human ovarian cancer-associated antigen OVA66. Methods The location of B-cell epitopes was predicted based on the physicochemical properties of amino acids including hydrophilicity, accessibility, polarity, and flexibility. This prediction was then combined with the result from ABCPred and screened via the secondary structure. Result The predicted B-cell epitopes of OVA66 were found to locate at or to be adjacent to the protein sequence of amino acids 209-216, 266-275, 294-301, 362-368, and 391-396. Conclusion The finding is helpful for the research on the use of synthetic peptide antigen for the early diagonosis of cancer.

关 键 词：卵巢肿瘤 T淋巴细胞 细胞毒性 表位 B淋巴细胞 抗原 肿瘤相关 碳水化合物 

分 类 号：R737.31[医药卫生—肿瘤] R392[医药卫生—临床医学]