藏灵菇源酸奶复合菌发酵剂对大鼠降胆固醇的研究  

STUDY ON THE CHOLESTEROL-REDUCING EFFECTS OF COMPLEX BACTERIA LEAVEN FROM KEFIR GRAIN MILK YOGHURT IN RATS

作　　者：冉冉[1] 王世平[1] 刘慧[2] 

机构地区：[1]中国农业大学食品科学与营业工程学院,北京100083 [2]北京农学院食品科学系,北京102206

出　　处：《营养学报》2009年第1期59-62,共4页Acta Nutrimenta Sinica

基　　金：北京市自然科学基金资助项目(No.5062006);北京市重点建设学科支持项目

摘　　要：目的探讨藏灵菇源酸奶复合菌发酵剂对实验性高胆固醇血症大鼠降胆固醇作用。方法从藏灵菇中筛选高效降胆固醇及产胆盐水解酶的三种乳酸菌株制备全脂和脱脂酸奶复合菌发酵剂。采用高脂大鼠模型,根据血清总胆固醇(TC)、甘油三酯(TG)及体重水平,将40只Wistar大鼠随机分成4组:对照组、高脂模型组、实验Ⅰ组(脱脂酸奶复合菌发酵剂组)和实验Ⅱ组(全脂酸奶复合菌发酵剂组)。对照组喂基础饲料,高脂模型组和实验Ⅰ、Ⅱ组饲喂高脂饲料,实验Ⅰ、Ⅱ组灌胃受试物,对照组和模型组给予蒸馏水,连续8w。在试验第2、4、6、8w分别采血,分离血清,检测TC、TG、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C)水平。结果实验Ⅰ组和实验Ⅱ组与高脂模型组相比,血清TC、TG、LDL-C含量降低非常显著(P<0.01)和显著(P<0.05)降低功效,而血清HDL-C含量有一定程度升高作用。降胆固醇效果实验Ⅰ组优于实验Ⅱ组。结论藏灵菇源酸奶复合菌发酵剂对实验性高胆固醇血症大鼠血清有显著的降胆固醇作用。Objective To investigate the cholesterol-reducing effects of complex bacteria leaven （CBL） from Kefir Grain milk yoghurt in experimental hypercholesterolemia rats. Method Isolated three strains of Lactobacilli from Kefir Grain milk yoghurt with high activity of bile salf hydrolase and cholesterol-reducing effect and prepare the CBL. Forty rats were randomly divided into four groups： control group, hypercholesterolemia model group, and experimentalⅠ, Ⅱ groups according to serum TC, TG, HDL-C and LDL-C concentrations. Control group was fed basic feed and three other groups were fed high fat feed. Experimental groups were given i.g. the test substances for 8 w, and control and model groups were given water, then serum TC, TG , HDL-C and LDL-C concentrations were determined at the end of the 2nd, 4th, 6th and 8th w. Results Compared with model group, serum TC, TG and LDL-C in experimentalⅠgroup and Ⅱgroup were significantly decreased and HDL-C increased after 8 w of treatments respectively, groupⅠ better than groupⅡ. Conclusion Complex bacteria leaven from Kefir Grain show significant effects of cholesterol-reducing effect in experimental hypercholesterolemia rats.

关 键 词：藏灵菇源酸奶 复合菌发酵剂 高胆固醇血症 降胆固醇 

分 类 号：R151[医药卫生—营养与食品卫生学]