青蒿琥酯对人前列腺癌PC-3细胞内游离Ca^(2+)的影响  

Effects of Artesunate on Free Ca^(2+) Concentration in Human Prostatic Cancer PC-3 Cells

作　　者：张长城[1] 张小鹏[1] 

机构地区：[1]三峡大学医学院植物与天然产物研究与利用湖北省重点实验室,宜昌市443002

出　　处：《中国药房》2009年第6期418-419,共2页China Pharmacy

基　　金：湖北省自然科学基金项目(2007ABA404);湖北省卫生厅2007~2008年度科研基金项目(JX3B31)

摘　　要：目的:研究青蒿琥酯(ART)对人前列腺癌PC-3细胞内游离Ca2+浓度([Ca2+]i)的影响。方法:ART处理PC-3细胞,用Ca2+荧光探针Fluo-3/AM标记PC-3细胞,激光共聚焦扫描显微镜检测PC-3细胞内[Ca2+]i变化。结果:ART诱导PC-3细胞内[Ca2+]i在0～0.5h显著升高;在0.5～1h维持高水平;在4～24h降到初始水平。结论:ART能够诱导PC-3细胞内[Ca2+]i显著持续升高,[Ca2+]i升高可能在ART诱导PC-3细胞凋亡中起重要作用。OBJECTIVE： To investigate the effects of artesunate（ART） on free Ca^2＋ concentration（ [Ca^2＋] i） in human prostatic cancer PC - 3 cells .METHODS：PC - 3 ceils were treated with ART and labeled using Ca^2＋ fluorescent probe Fluo - 3/ AM, and the change of [ Ca^2＋ ]i was evaluated by confocal laser scanning microscopy. RESULTS： After PC - 3 cells were exposed to artesunate, the intracellular [Ca^2＋]i concentration increased significantly at 0-0.5 h,maintained at a high level at 0.5-1 h, but decreased to the level of the initial at 4-24 h. CONCLUSION： ART can induce the marked sustained increase of[Ca^2＋] i and [ Ca^2＋]i might play an important role in ART - induced apoptosis of human PC - 3 cells.

关 键 词：青蒿琥酯 前列腺癌 游离CA^2+浓度 

分 类 号：R737.25[医药卫生—肿瘤] R966[医药卫生—临床医学]