高效液相色谱法测定复方茶碱麻黄碱片中可可碱的含量  

Determination of Theobromine in Compound Theophylline and Ephedrine Tablet by HPLC

作　　者：刘海侠[1] 吴庆淼[1] 

机构地区：[1]松原市药品检验所,吉林松原138000

出　　处：《中国中医药信息杂志》2009年第3期49-50,共2页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的建立复方茶碱麻黄碱片中可可碱的含量测定方法。方法采用高效液相色谱法,以KromasilC18(4.6mm×250mm,5μm)为色谱柱;流动相为0.05mol/L磷酸二氢钾溶液-甲醇-三乙胺(77∶23∶0.2);检测波长为215nm;柱温为45℃;流速为0.8mL/min。结果可可碱在0.3094～0.722μg(r=0.99996)浓度范围内呈良好的线性关系,平均回收率为100.49%,RSD=1.1%。结论本法可准确、灵敏、快速地测定复方茶碱麻黄碱片中可可碱的含量。Objective To establish a HPLC method for determination of theobromine in Compound Theophylline and Ephedrine Tablet. Method HPLC method was adopted on Kromasil C18 column （250 mm×4.6 mm, 5 μm）, and temperature was set at 45 ℃. The mobile phase consisted of 0.05 mol/L KH2PO4- Methanol- Triethanolamine （77 ： 23 ： 0.2）, with flow rate of 0.8 mL/min. The UV detection wavelength was set at 215 nm. Results The calibration curves of theobromine was linear in the range of 0.3094- 0.722 ℃g （r=0.999 96）, ant the average recovery was 100.49% （n=9） with RSD=1.1%. Conclusion This method is simple, rapid, sensitive and accurate, and can be used for determination of theobromine in Compound TheophyUine and Ephedrine Tablet.

关 键 词：复方茶碱麻黄碱片 高效液相色谱法 可可碱 含量测定 

分 类 号：R284.1[医药卫生—中药学]