盐酸氯丙嗪-联吡啶钌体系在多壁碳纳米管修饰电极上的电化学发光行为研究及盐酸氯丙嗪的测定  

Study on Electrochemiluminescence Behaviors of Chlorpromazine Hydrochloride-Tris(2,2'-bipyridyl)ruthenium(Ⅱ) System at Multiwall Carbon Nanotubes Modified Electrode

作　　者：李利军[1] 陈昌东[2] 程昊[1] 蔡卓[2] 钟亮[2] 李斯光[2] 吴健玲[2] 

机构地区：[1]广西工学院生物与化学工程系,广西柳州545006 [2]广西大学化学与化学工程学院,广西南宁530004

出　　处：《分析测试学报》2009年第1期37-43,共7页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(20665001);广西科学基金资助项目(0640029)

摘　　要：在玻碳电极上成功制备了多壁碳纳米管修饰电极(MWCNTs/GCE),优化了该修饰电极的制备条件。研究了联吡啶钌和盐酸氯丙嗪在该修饰电极上的电化学行为和电化学发光行为,建立了电化学发光法测定尿液中盐酸氯丙嗪的分析方法。结果表明,联吡啶钌-氯丙嗪体系在MWCNTs/GCE上表现出很好的电化学活性和电致化学发光响应,多壁碳纳米管不但增大了玻碳电极的比表面积而且加快了联吡啶钌在电极表面的电化学氧化,对联吡啶钌的电化学发光强度具有明显的增敏作用,同时盐酸氯丙嗪对联吡啶钌在该修饰电极上的电致化学发光具有很强的增敏作用。在0.1mol/L的磷酸盐(pH7.5)缓冲溶液中,盐酸氯丙嗪在该修饰电极上的检出限(S/N=3)为6.0×10-7mol/L,在1.0×10-6～4.0×10-4mol/L范围内浓度与相对发光强度呈线性关系(r=0.9952)。连续测定6.0×10-5mol/L的盐酸氯丙嗪溶液13次,发光强度的RSD值为2.50%,表明该修饰电极具有较好的重复性。该方法已经成功地应用于尿样的检测。A multiwall carbon nanotubes modified glassy-carbon electrode（MWCNTs/GCE） was pre- pared at the bare GCE. The electrochemical and electrochemiluminescencial behaviors of chlorproma- zine hydrochloride（CPZ） and Ru（bpy）3^21＋ at the modified electrode were studied. The results indica- ted that Ru （bpy）3^21＋ exhibited a good electrochemical activity and ECL response at the modified elec- trode, in which MWCNTs not only enlarged electroactive specific surface area of electrode, but also accelerated the rate of electron transfer between the redox-active Ru （bpy） 3^21＋ and the electrode. The addition of CPZ would further lead to an enhancement of ECL intensity of Ru （bpy） 3^21＋ Based on this, an electrochemiluminescence method for determination of CPZ was established. Under optimal conditions, the calibration curve showed a good linearity in the range of 1.0 × 10^-6 -4. 0 × 10^-4 mol/L with a detection limit（ S/N= 3 ） of 6.0 × 10^-7 mol/L. The RSD for determination of 6. 0× 10^-5 mol/L CPZ（n = 13） was 2.50%. The presented method had the advantage of good repeatibility and sensitivity, and was applied in the determination of CPZ in urine samples with satisfactory results.

关 键 词：多壁纳米碳管 修饰电极 联吡啶钌 盐酸氯丙嗪 电致化学发光 

分 类 号：O657.15[理学—分析化学] R971.4[理学—化学]