感染根管机械预备联合不同化学方法预备后细菌残余量的实时定量检测  

Quantitative evaluation of residual endodontic microorganisms after mechanical root canal preparation different chemical preparations by real-time PCR

作　　者：姜云涛[1] 夏文薇[1] 梁景平[1] 马善奋[1] 闫培芳[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院·口腔医学院牙体牙髓科.上海市口腔医学重点实验室,上海200011

出　　处：《上海口腔医学》2009年第1期10-14,共5页Shanghai Journal of Stomatology

基　　金：上海市卫生局资助项目(2007012);上海市教育委员会资助项目(jdy-07060)~~

摘　　要：目的:探讨快速、敏感定量检测感染根管病原菌的方法;并在RNA水平评估不同根管化学预备方法清除根管细菌的效果。方法:选择慢性根尖周炎单根管患牙24颗,随机分为实验组与对照组。常规根管机械预备后,对照组用3%H2O2、1%NaClO交替冲洗根管;实验组加用EDTA凝胶根管化学预备,联合3%H2O2、1%NaClO交替冲洗。预备前后根管采样,提取总RNA并反转录为cDNA,进行实时PCR定量分析,采用SAS6.12软件包对数据进行方差分析。结果:机械预备联合化学预备后,根管内细菌数量显著降低(P<0.01);其中实验组清除病原菌的效果显著优于对照组(P<0.05)。结论:实时PCR定量检测技术可以快速、敏感的检测根管内病原菌,机械预备联合化学预备可有效降低根管内细菌的数量。PURPOSE： To establish a quick, sensitive method for quantifying root canal flora and investigate the effects of different root canal preparations on the pathogenic bacteria at RNA level. METHODS： A total of 24 single-rooted teeth with chronic apical periodontitis were selected and prepared using 3% H2O2 combined with 1% NaClO, EDTA combined with 3% H2O2,1% NaClO,respectively,the samples were taken before and after root canal preparation. After isolation of total RNA from the root canal samples, cDNA was synthesized by reverse transcription, and detected by realtime PCR. The data were analyzed with SAS 6.12 software package. RESULTS： The number of bacteria in the root canal reduced dramatically after mechanical preparation and irrigated using 3% H2O： and 1% NaClO（P〈0.01 ）. Further combined with EDTA, its effect was better than that of simply irrigated using 3% H2O and 1% NaClP（P〈0.05）. CONCLUSIONS： Real-time PCR can be employed in the identification of bacteria flora in the root canal, both methods of root canal preparation can effectively reduce the number of bacteria flora.

关 键 词：慢性根尖周炎 实时聚合酶链反应 RNA 根管预备 

分 类 号：R781.341[医药卫生—口腔医学]