树鼩实验性肝癌过程中GGT酶阳性肝细胞灶、IGF-Ⅱ及HBxAg异常表达  

Expression of GGT、insulin-like growth factor Ⅱ and HBxAg in precancerous events of hepatocarcinogenesis induced by AFB1 and/or HBV in Chinese Tree Shrews 

作　　者：覃柳亮 苏建家 杨春 李瑗 班克臣 黄国华 罗小玲 段小娴 严瑞琪[2] 

机构地区：[1]广西肿瘤研究所病理研究室 [2]中山医科大学肿瘤防治中心

出　　处：《广西医学》1998年第1期4-7,共4页Guangxi Medical Journal

摘　　要：动态观察ＨＢＶ与ＡＦＢ１诱发树肝细胞癌（ＨＣＣ）病变过程中肝癌前病变γ－谷氨酰转肽酶阳性肝细胞灶（ＧＧＴ阳性灶）、胰岛素样生长因子Ⅱ（ⅠＧＦ－Ⅱ）发生情况，探讨ＨＣＣ发生的可能机制。实验动物分４组，Ａ组：ＨＢＶ阳性和摄入ＡＦＢ１；Ｂ组：ＨＢＶ阳性组；Ｃ组：摄入ＡＦＢ１；Ｄ组：空白对照。组织化学及免疫组织化学检测肝活检组织。结果：（１）肝癌前病变ＧＧＴ阳性灶在各实验组不仅灶数量，而且灶大小均明显高于空白组（Ｐ＜０．０５），且双诱癌因素组（Ａ）灶数量明显大于单诱癌因素组（Ｂ、Ｃ）（Ｐ＜０．０５）；（２）实验第１５周，即有ＩＧＦ－Ⅱ阳性表达，在第４５周阳性率明显高于第７５周（Ｐ＜０．０５）；（３）ＨＢＶ感染组（Ａ，Ｂ）动物肝组织中ＨＢｓＡｇ阳性率高达９２．１％，首次在树肝组织中检出ＨＢｘＡｇ，且阳性率达８６．５％。提示：ＨＢＶ与ＡＦＢ１协同致树ＨＣＣ；ＨＣＣ发生早期就有ⅠＧＦ－Ⅱ异常表达。Studies on the expressions of GGT, insulin-like growth factor Ⅱ(IGF-Ⅱ) and HBxAg during the development of hepatocellular carcinoma (HCC) induced by Hepatitis B Virus (HBV) and Aflatoxin B1 (AFB1) was done in animal model of Tree Shrew 120 tree shrews were divided into 4 groups Group A with HBV-infection were fed with AFB1; group B was HBV-infection alone; group C was exposed to AFB1 alone; group D used as a control The expression of oncogenes and HBxAg were detected with immunohistochemical assay γ-glutamyltranspeptidas(GGT) positive foci in group A was more than that in group B and C (P<005) The expression level of IGF-Ⅱ at the 45th week was higher than that at the 75th week in group A, B and C(P<005) The high level of IGF-Ⅱ expression appeared as early as at the 15th week HBsAg and HBxAg were detected in the HBV-infection groups (A and B) The results showed that (1) Synergistic hepatocarcinogenic effect of HBV infection and AFB1 exposure existed in this Tree Shrew model (2) IGF-Ⅱ was an early marker in hepatocarcinogenesis

关 键 词：肝肿瘤 谷氨酰转肽酶 HBXAG IGF-Ⅱ 

分 类 号：R735.7[医药卫生—肿瘤]