乙烯对‘洛阳红’牡丹切花开放和衰老进程及内源乙烯生物合成的影响  

Effect of Ethylene on Cut Flowers of Tree Peony ‘Luoyang Hong’ Opening and Senescence Process and Endogenous Ethylene Biosynthesis

作　　者：周琳[1] 贾培义[1] 刘娟[1] 王玮然[1] 霍志鹏 董丽[1] 

机构地区：[1]北京林业大学园林学院,国家花卉工程技术研究中心,北京100083 [2]河南省洛阳市花木公司,河南洛阳471000

出　　处：《园艺学报》2009年第2期239-244,共6页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(30571309);教育部新世纪优秀人才支持计划项目(NCET-05-0138)

摘　　要：以开花指数为1级的‘洛阳红’牡丹切花为试材,研究了外源乙烯和乙烯作用抑制剂1-甲基环丙烯(1-MCP)处理对其采后开花衰老进程中开花指数、花径增大率、瓶插寿命、内源乙烯生成量及乙烯生物合成关键酶ACC合成酶(ACS)和ACC氧化酶(ACO)活性的影响,从乙烯生物合成角度探讨了乙烯对其采后开花衰老的调节机理。结果表明,10μL.L-1乙烯处理6h明显加快了‘洛阳红’花朵开放进程,缩短了切花的瓶插寿命,并促使其在盛开后出现严重落瓣;1.0μL.L-11-MCP处理6h则延缓了花朵开放,延长了瓶插寿命,但却影响了部分切花的充分开放;内源乙烯的生成分别受乙烯和1-MCP处理的促进和抑制,与切花的开放衰老进程密切相关。不同处理后ACS、ACO酶活性分析表明,ACS活性变化与内源乙烯的生成相联系,是影响牡丹切花开放衰老进程的主要因子,这与目前得出的ACS是植物乙烯生物合成限速酶的研究结果相一致。Tree peony cut flower （Paeonia suffruticosa ＇ Luoyang Hong＇ ） harvested in the first grade of flower opening index was taken as material to investigate the influence of exogenous ethylene and ethylene action inhibitor 1-MCP on flower opening index, diameter increasing rate, vase life, endogenous ethylene production and ethylene biosynthetic enzymes activities of ACC synthase （ACS） and ACC oxidase （ACO） during postharvest opening and senescence. The results showed that 10 μL · L^-1 ethylene treatment for 6 h hastened the process of flower opening, shortened vase life and induced petals abscission seriously after full-open; While 1.0 μL · L^-1 1-MCP retarded flower opening, prolonged flower vase life and impeded full-open of some flowers. Endogenous ethylene production was promoted by ethylene and inhibited by 1-MCP respectively, which was closely associated with the flower opening process. ACS and ACO activities analysis after different treatments indicated that changes in ACS activity were related to the endogenous ethylene production, suggesting that ACS be the main factor in the regulation of tree. peony cut flower opening and senescence, which confirmed the former conclusion that ACS is the rate-limiting enzyme in plants ethylene biosynthesis.

关 键 词：牡丹 切花 乙烯 1-MCP 开花 衰老 ACS酶活性 ACO酶活性 

分 类 号：S685.11[农业科学—观赏园艺]