松实小卷蛾雌蛾对思茅松针叶挥发性化合物的触角电位反应  

Electroantennogram Response of Female Moth of Retinia cristata to Volatile Terpenes from Needles of Pinus kesiya var.langbianensis

作　　者：闫争亮[1,2] 胡光辉[1] 冯志伟[1] 马惠芬[1,2] 

机构地区：[1]云南省林业科学院,云南昆明650204 [2]国家林业局云南珍稀濒特森林植物保护和繁育重点实验室,云南省森林植物培育与开发利用重点实验室,云南昆明650204

出　　处：《安徽农业科学》2009年第3期1153-1154,1162,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(30560125);云南省自然科学基金项目(2005-C0065M)

摘　　要：[目的]研究思茅松针叶气味对松实小卷蛾产卵行为的影响。[方法]室内验证松实小卷蛾产卵是否受到寄主松树气味的影响,进行室内行为测试和触角电位实验。[结果]结果表明,保留触角的松实小卷蛾雌蛾在新鲜针叶上的产卵量大于在枯萎针叶上的量,而切除了触角的雌蛾则对新鲜和枯萎针叶没有选择性。松实小卷蛾雌蛾对思茅松针叶挥发性物质主要成分α-蒎烯、β-蒎烯、月桂烯、柠檬烯、β-水芹烯都表现出一定的触角电位反应,以β-水芹烯引起的松实小卷蛾触角电位反应最大,其次是柠檬烯。[结论]β-水芹烯、柠檬烯在新鲜针叶挥发物中的含量远大于在枯萎针叶挥发物中的含量,β-水芹烯、柠檬烯可能是刺激松实小卷蛾雌蛾产卵的主要物质。[ObjeCtive] The purpose was to study the effect of volatile terpenes from needles of Piruts kesiya var. langbianensis to oviposition behavior of Retinia cristata. [Method] Whether the oviposition behavior of Retinia cristata was influenced by Piruts kesiya var. langbianensis odour was validated in room, and the electroantennogram experinment was made. [Result]The results indicated that normal moths deposited much raore eggs on fresh pine needles than on withered needles; however, antennectomized moths did not exhibit any selectivity. Electroantennogram （EAG） study showed that the female moths had stated response to main compositions of needle volatile of α-pinene,β-pinene, myrcene, limonene and β-phellandrene, β-phellandrene elicited the largest EAG response, and limonene was secondly. [ Conclusion]Taking into account the fact that there is mare β-phellandrene and limanene in volatile of fresh needles than that of withered needles, the two compounds are speculated as the primary oviposition stimulants.

关 键 词：松实小卷蛾 雌蛾 思茅松 挥发性化合物 触角电位反应 

分 类 号：S763.3[农业科学—森林保护学]