没食子酸抗HBsAg/HBeAg的实验研究  

March the Magnificent Goal of Crossing New Century

作　　者：郑民实[1] 孔庚星[2] 张鑫[2] 陈楚城[2] 段文军[2] 夏志祥[2] 

机构地区：[1]江西医学院,330006 [2]中国人民解放军广州医学高等专科学校

出　　处：《实用中医药杂志》1998年第1期5-7,共3页Journal of Practical Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(No.39370828)

摘　　要：使用酶联免疫吸附检测（ＥＬＩＳＡ）技术对没食子酸水溶液进行抗ＨＢｓＡｇ和ＨＢｅＡｇ的实验研究。本法是通过Ｐ／Ｎ比值的变化判定药效。若按５种剂量（０．３０、０．６０、１．２０、２．５０、５．００ｍｇ／１００μｌ）的药物，２种不同浓度（１０．９２、１４．２６Ｐ／Ｎ比值）的ＨＢｓＡｇ／ＨＢｅＡｇ与３种不同接触时间（立即、１小时、２小时）的１０项Ｐ／Ｎ比值的均数来综合评价药效指数时，５种抗ＨＢｓＡｇ药物的排列次序为《没食子酸》（４．８６）＞（优于，下同），《云芝肝泰》（５．５３）＞《乙肝冲剂》（６．１１）＞《乙肝宁》，（６．６６）以及抗ＨＢｅＡｇ药物的排列次序为《乙肝宁》（３．９２）＞《乙肝冲剂》（４．８７）＞《没食子酸》（５．４８）＞《云芝肝秦》（５．５１）；若按上述抗ＨＢｓＡｇ和抗ＨＢｅＡｇ各１０项Ｐ／Ｎ比值的均数来综合评价药效指数时，４种抗乙型肝炎药物的排列顺序为《没食子酸》（５．１７）＞《乙肝宁》（５．２９）＞《乙肝冲剂》（５．４９）＞《云芝肝泰》（５．５２）。由此可见，《没食子酸》是实验室筛出的抗 ＨＢｓＡｇ和抗ＨＢｅＡｇ有效的药物。Engyme Linked Immunosorbent As- say (ELISA) was used to engage the experieuce or anti - HBsAg and anti - HBeAg in water solutiou of gallic acid. The drug effect was judged according to the change in P/N ratio. When it was judged n different dosage, concentration and touching time in P/N ratio, gallic acid was ranked the first one in the five anti- HBsAg drugs. However, When it was judged in anti- HBsAg and anti-HBeAg in P/N ratio, gallic acid was also ranked the first on in the four drugs of anti -B hepatitis. It showed that gallic acid is the effective drug in anti - HBsAg aud anti - HBeAg.

关 键 词：没食子酸 抗HBSAG 抗HBeAg 实验研究 中药 

分 类 号：R285.5[医药卫生—中药学]