大黄鱼gdf-8Ⅱ开放阅读框序列克隆及分析  

Molecular Cloning and Analysis of gdf-8 Ⅱ Open Reading Frame from Pseudosciaena crocea

作　　者：孙升[1] 薛良义[1] 童丽娟[1] 

机构地区：[1]宁波大学应用海洋生物技术教育部重点实验室,浙江宁波315211

出　　处：《宁波大学学报（理工版）》2009年第1期39-43,共5页Journal of Ningbo University：Natural Science and Engineering Edition

基　　金：国家863计划项目(2006AA10A405);浙江省自然科学基金(Y306295);宁波市自然科学基金(2006A610083);浙江省教育厅新苗人才计划项目(2007R40G2070027);宁波大学科研基金(XK0613041)

摘　　要：GDF-8是动物肌肉发育和生长过程中的负调控因子,对gdf-8的研究将有助于促进重要养殖鱼类大黄鱼的遗传育种.在已克隆大黄鱼gdf-8 Ⅰ的基础上,采用同源克隆策略,首次克隆了2 357 bp的大黄鱼gdf-8 Ⅱ开放阅读框序列(ORF),它由3个外显子、2个内含子组成,编码359个氨基酸,其中外显子Ⅲ与大黄鱼gdf-8 Ⅰ的外显子Ⅲ序列相似度较高,这提示外显子Ⅲ受到了高度的进化限制及其功能的重要性.基于gdf-8推导的氨基酸序列构建的系统树显示鱼类的gdf-8 Ⅱ是与gdf-8 Ⅰ不同的基因,有必要对gdf-8 Ⅱ基因进行进一步的研究.The GDF-8, a negative regulator of myogenesis, inhibits myoblast proliferation and differentiation. The studies of its gdf-8 will be helpful to investigating the genetic breeding of marine cultured fish Pseudosciaena crocea. Three pairs of primers are designed to clone gdf-8 Ⅱ open reading frame （ORF） by adopting the strategy of homology cloning. The ORF is 2 357 bp in length, including 3 Exons and 2 Introns. It encodes 359 amino acids. The Exon Ⅲ ofgdf-8 Ⅱ is highly similar to that ofgdf-8 Ⅰ, suggesting that it is much restricted in the evolution and its function is highly conservative. The phylogenetic tree, which is constructed on the basis of the amino acid derived from gdf-8, shows the difference ofgdf-8 Ⅱ from gdf-8 Ⅰ in fish, suggestive of further study gdf-8 Ⅱ.

关 键 词：大黄鱼 gdf-8Ⅱ 开放阅读框 克隆 

分 类 号：Q75[生物学—分子生物学]