用SRAP标记鉴定落羽杉属植物杂种  

Identification of Taxodium Hybrids by SRAP Analysis

作　　者：於朝广[1] 殷云龙[1] 徐建华[1] 

机构地区：[1]江苏省·中国科学院植物研究所(南京中山植物园),南京210014

出　　处：《林业科学》2009年第2期142-146,共5页Scientia Silvae Sinicae

基　　金：江苏省高技术(农业)研究项目(BG2007318);国家林业局植物新品种保护办公室植物新品种测试指南及已知品种数据库项目(2007010);江苏省林业三项工程项目(lysx200702)

摘　　要：In this paper,the authenticity of 4 reciprocal progenies from Taxodium distichum and T.mucronatum was identified by SRAP(Sequence-related Amplified Polymorphism) markers.Firstly,48 SRAP primer combinations were randomly selected and polymorphism primers between two parents(T.distichum and T.mucronatum) were selected.As a result,all primers amplifid clear bands,and 29 primers had polymorphism bands of two parents,accounted for 60.4% of all primers,and 14 primer combinations with specific bands both two samples.Authenticity of 4 progenies from T.distichum and T.mucronatum was identified by 12 polymorphism primer combinations selected from above 29 polymorphism primer combinations.The results showed that 4 progenies were all identified to be true hybrids owing to their specific bands from male parent.Among the primer combinations,Me9+Em10 produced specific bands of both male parent and female parent in every progeny,that is to say,with this one primer combination alone,the status of 4 progenies can be identified.Efficiency of molecule identification were much improved.The results of hybrids identification provided a solid evidence for the hybrids further study and application.The experimental results also proved that SRAP molecular marker technology can be effectively applied in the researches on Taxodium.In this paper,the authenticity of 4 reciprocal progenies from Taxodium distichum and T. mucronatum was identified by SRAP （Sequence-related Amplified Polymorphism） markers. Firstly, 48 SRAP primer combinations were randomly selected and polymorphism primers between two parents （ T. distichum and T. mucronatum ） were selected. As a result, all primers amplifid clear bands, and 29 primers had polymorphism bands of two parents, accounted for 60.4 % of all primers, and 14 primer combinations with specific bands both two samples. Authenticity of 4 progenies from T. distichum and T. mucronatum was identified by 12 polymorphism primer combinations selected from above 29 polymorphism primer combinations. The results showed that 4 progenies were all identified to be true hybrids owing to their specific bands from male parent. Among the primer combinations, Me9 ＋ Em10 produced specific bands of both male parent and female parent in every progeny, that is to say, with this one primer combination alone, the status of 4 progenies can be identified. Efficiency of molecule identification were much improved. The results of hybrids identification provided a solid evidence for the hybrids further study and application. The experimental results also proved that SRAP molecular marker technology can be effectively applied in the researches on Taxodium .

关 键 词：落羽杉属 SRAP-PCR 杂种鉴定 

分 类 号：S718.46[农业科学—林学] S718.49