应用胃癌组织芯片研究RUNX3、VEGF与MVD的相关性及其意义  

Correlation analysis among RUNX3,VEGF and MVD and their significance using gastric cancer tissue microarray

作　　者：伏洁[1] 张煦[1] 王靖[1] 

机构地区：[1]兰州大学基础医学院病理学研究所,甘肃省兰州市730000

出　　处：《世界华人消化杂志》2009年第3期321-325,共5页World Chinese Journal of Digestology

摘　　要：目的:探讨胃癌组织芯片中RUNX3、VEGF表达与MVD的相关性及其意义.方法:采用组织芯片技术建立经确证80例胃癌的组织芯片,免疫组化检测芯片中RUNX3、VEGF的表达及微血管密度计数.结果:RUNX3在胃癌芯片中表达阳性率分别为阴性46.3%(37/80)、弱阳性35.0%(28/80)和强阳性18.7%(15/80);VEGF的表达阳性率分别为阴性22.5%(18/80)、弱阳性30.0%(24/80)和强阳性47.5%(38/80),其阳性表达部位为细胞质.MVD值RUNX3表达阳性者显著低于表达阴性者;VEGF表达阳性者显著高于表达阴性者,RUNX3的表达和MVD之间存在显著负相关(rs=-0.742,P<0.01),VEGF的表达和MVD之间存在显著正相关(rs=0.683,P<0.01).VEGF表达阳性率在RUNX3阴性组逐渐增高;在RUNX3弱阳性组逐渐增高,在RUNX3强阳性组逐渐降低,RUNX3的表达和VEGF之间存在显著的负相关(rs=-0.333,P<0.01).结论:RUNX3作为一种抑癌基因可能通过某种机制抑制VEGF的表达减少胃癌的血管新生、生长和转移;联合检测RUNX3、VEGF和MVD的表达,对把握胃癌生物学行为和预后有一定的价值。AIM： To investigate the expressions of human runt-related transcription factor 3 （RUNX3）, vascular endothelial growth factor （VEGF） and microvascular density （MVD） using gastric cancer tissue microarray and their significance. METHODS： Gastric cancer tissue microarray was prepared, and the expressions of RUNX3, VEGF and MVD were detected using immunohistochemistry in 80 gastric cancer cases proved pathologically. RESULTS： RUNX3 expression was classified as negative 46.3% （37/80）, weak positive 35.0% （28/80） and strong positive 18.7% （15/80） respectively; VEGF was strongly expressed in 47.5% （38/80）, weakly in 30% （24/80） and negatively in 22.5% （18/80）. A positive reaction was mainly distributed in cytoplasm. MVD was lower in the positive expression of RUNX3 than the negative expression; MVD in the positiveexpression of VEGF was greater than the negative expression. The expression of RUNX3 was negatively correlated with MVD （rs = -0.742, P 〈 0.01）, and the expression of VEGF was positively correlated with MVD （rs = 0.683, P 〈 0.01）. The positive expression rates of VEGF increased gradually in negative expression and weak positive but decreased gradually in strong positive of RUNX3. The expression of RUNX3 was negatively correlated with VEGF （rs = -0.333, P 〈 0.01）. CONCLUSION： RUNX3 may inhibits the expression of VEGF by some mechanism and reduces the angiogenesis, growth, and metastasis of gastric cancer, combined detection of RUNX3, VEGF and MVD can estimate the biological behaviour and prognosis of the patients more exactly.

关 键 词：胃癌 组织芯片 人类相关转录因子3 血管 内皮生长因子 微血管密度 

分 类 号：R735.2[医药卫生—肿瘤]