微囊藻毒素和水体有机污染物联合诱导SHE细胞转化及相关癌基因的变化  

The Combined Effects of MCYST and Water Organic Pollutants on the Co induction of SHE Cell Transformation

作　　者：王红兵[1,2] 朱惠刚[1,2] 聂岚[1,2] 徐均康[1,2] 

机构地区：[1]北京医科大学劳动卫生学教研室 [2]上海医科大学环境卫生教研室

出　　处：《卫生研究》1998年第1期41-44,共4页Journal of Hygiene Research

基　　金：国家自然科学基金

摘　　要：应用ＳＨＥ细胞体外二阶段转化实验和免疫组化方法，分析了上海淀山湖水体有机污染物和微囊藻毒素（ＭＣＹＳＴ）联合诱导ＳＨＥ细胞恶性转化的作用，发现微囊藻毒素单独作用不能诱发ＳＨＥ细胞转化，但可明显促进低剂量水体有机污染物启动的细胞恶性转化。在典型的Ⅲ型转化灶细胞中ｒａｓＰ２１蛋白表达明显增强。提示：微囊藻毒素具有促癌作用，二阶段诱导的转化细胞中ｒａｓ癌基因被激活。he authors studied the combined carcinogenic activities of MCYST and organic pollutants in water of Dianshan Lake, using in vitro Syrian hamster embryo (SHE) cell transformation and immunohistochemical assays. The results showed that MCYST alone could not induce SHE cell transformation, but it increased the effects of low dose organic pollutants in cell transformation in a dose dependent manner. In the cells isolated from type Ⅲ transformed foci, ras P21 protein showed a positive immunohistochemical staining. It suggests MCYST may be a tumor promoter, which has a synergistic carcinogenic activity organic pollutants in water and the activation of ras oncogene may be one of the factors inducing malignant transformation.

关 键 词：微囊藻毒素 水体 SHE细胞转化 有机污染物 肿瘤 

分 类 号：X52[环境科学与工程—环境工程] R730.23[医药卫生—肿瘤]