几株紫色非硫细菌的分离鉴定与产氢特性分析  

Identification, Culture Characteristics and Hydrogen-producing Ability Analysis of Several Purple Non-sulfur Bacterial Strains

作　　者：王中康[1,2] 胡小容[1,2] 廖强[3] 叶姜瑜[4] 张宝鹏[1,2] 殷幼平[1,2] 

机构地区：[1]重庆大学重庆市功能基因及调控重点实验室,重庆400030 [2]重庆大学生物工程学院基因工程中心,重庆400030 [3]重庆大学动力工程学院,重庆400030 [4]重庆大学城市建设与环境工程学院,重庆400030

出　　处：《应用与环境生物学报》2009年第1期120-124,共5页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金重点项目(No.90510020)~~

摘　　要：从重庆地区不同环境淤泥、泥水样品中,经富集培养、分离纯化,获得5株紫色非硫细菌.根据菌体的菌落形态、染色特性、生理生化特征及活细胞光吸收峰对菌株进行常规鉴定,结合菌株16SrDNA扩增测序进行分子生物学分析验证,构建了菌株与数据库中近缘菌株的系统发育树.以优化的培养条件(营养、pH、接种量等参数)对供试菌株的生理生化特性和产氢能力做了比较分析.结果显示,5个菌株均为光合产氢细菌,菌株AN1、D1为沼泽红假单胞菌(Rhodopseudomonas palustris),AN2、AS1、BS1等3株为类球红细菌(Rhodobacter sphaeroides);其中类球红细菌菌株AN2在给定的培养条件下光合产氢能力最高,可达9.55μg/mLd-1,是一株有应用前景的光合产氢细菌.Several purple non-sulfur bacteria, collected from aeration basin mud, pond mud and turbid water in Chongqing, China, were screened and identified based on their morphological, physiological and biochemical characteristics, cellular spectra, and 16S rDNA sequences. Their culture characteristics and hydrogen-producing ability were determined also. The results showed that two of the 5 isolated strains, AN1 and D1, were identified as Rhodopseudomonaspalustris and the other 3, AN2, AS1 and BS1, belonged to Rhodobacter sphaeroide. The study on the culture characteristics showed that there were many differences among the 5 isolates in carbon utilization abilities, optimum culture conditions and hydrogen-producing abilities. Isolate AN2 （R. sphaeroide） was provided with the highest hydrogen-producing ability under the given culture conditions. Fig 4, Tab 2, Ref 15

关 键 词：紫色非硫细菌 鉴定 生理生化特性 光合产氢 沼泽红假单胞菌 类球红细菌 

分 类 号：Q93[生物学—微生物学]