液化石油气中毒大鼠的行为学与病理研究  

Behavioral and pathological observation of liquid petroleum gas poisoning in rats

作　　者：白润涛[1] 李竞[1] 葛朝莉[1] 韩漫夫[1] 

机构地区：[1]广东深圳市第二人民医院神经内科,深圳518035

出　　处：《中国实用神经疾病杂志》2009年第2期8-10,共3页Chinese Journal of Practical Nervous Diseases

基　　金：深圳市科技计划基金资助项目(200802040)

摘　　要：目的观察大鼠急性液化石油气中毒后行为学和病理变化。方法采用大鼠液化石油气中毒模型、水迷宫实验观察大鼠逃避潜伏期、在无平台水迷宫中第1次穿越平台所在位置的时间和在平台所在象限游泳距离,实验大鼠分别于中毒后2、3、5d处死取脑,HE染色观察神经细胞形态。结果水迷宫实验中中毒组动物逃避潜伏期明显延长,在无平台水迷宫中第1次穿越平台所在位置的时间明显延长,在平台所在象限游泳距离缩短。光镜下见中毒组大鼠神经元细胞核浓缩、结构不清。结论液化石油气中毒导致神经元细胞坏死,从而损害大鼠的学习记忆认知功能。Objective To observe the behavioral and pathological changes of liquid petroleum gas poisoning rat. Methods Liquid petroleum gas poisoning model of rats were used. The escape latency and the first time passing hidden platform and the distance of swimming in the quadrant of hidden platform were recorded through Morris water maze task. The rats were killed at 2,3,5d after poisoning. The morphological changes of neurocyte were observed by HE staining. Results The escape latency increased significantly in poisoning rats. The first time passing hidden platform prolonged significantly and the distance of swimming in the quadrant of hidden platform decreased significantly in poisoning rats in Morris water maze task. The degeneration of neurons and inspissation of karyon were observed by light microscope. Conclusion Liquid petroleum gas poisoning can result neurons necrosis and damage the learing and memory and cognition abilities in rat.

关 键 词：液化石油气中毒 MORRIS水迷宫 神经细胞 大鼠 

分 类 号：R-332[医药卫生]