一株异养脱氮菌的脱氮性能及其影响因素研究  

Study on nitrogen removal of a heterotrophic nitrification removal bacteria strain and its affecting factors

作　　者：黄珏[1] 何义亮[1] 赵彬[1] Shauna Taylor 张文英[1] 

机构地区：[1]上海交通大学环境科学与工程学院,上海200240

出　　处：《环境工程学报》2009年第3期395-399,共5页Chinese Journal of Environmental Engineering

基　　金：国家自然科学基金资助项目(20676078)

摘　　要：研究异养硝化过程中分离得到一株具有异养脱氮性能的菌株,经16S rRNA鉴定后为Providencia rettgeri菌属,命名为Providencia rettgeristrain YL。在实验室条件下,初步探讨了不同碳氮源、温度、pH值、摇床转速和微量元素对Providencia rettgeristrain YL菌株脱氮作用的影响,研究结果显示,Providencia rettgeristrain YL菌株在葡萄糖和氯化铵为碳氮源的培养基中生长最好,温度为30℃、pH值为7、摇床转速为120 r/min时具有最佳的脱氮效果,微量元素Mg2+最有利于Providencia rettgeristrain YL菌株的生长和脱氮。A new strain with the ability of heterotrophic nitrogen removal was isolated. Through 16 s rRNA gene sequencing analysis,the bacteria was related to Providencia rettgeri and there after named Providencia rettgeri strain YL. Varying carbon and nitrogen sources, temperature, pH, shaking speeds and microelements were investigated to identify the optimal conditions for nitrogen removal. The results were as follows： temperature 30 ℃ , pH 7, shaking speed 120 r/min, the presence of magnesium ions. Glucose and ammonium chloride were the best carbon and nitrogen sources.

关 键 词：PROVIDENCIA rettgeri STRAIN YL 脱氮条件 异养脱氮 

分 类 号：X172[环境科学与工程—环境科学]