淋巴瘤血管内皮细胞人白细胞DR抗原和第Ⅷ因子相关抗原的表达  

Expression of class Ⅱ major histocompatibility antigen and factor Ⅷ related antigen in endothelial cells of blood vessels in malignant lymphomas

作　　者：吴后男[1,2,3,4,2] 李玉林[1,2,3,4,2] 申哲洙[1,2,3,4,2] 

机构地区：[1]白求恩医科大学病理学教研室 [2]解放军第二二二医院 [3]北京儿童医院 [4]第一临床医学院普外科

出　　处：《中华病理学杂志》1998年第1期27-30,共4页Chinese Journal of Pathology

摘　　要：目的试图揭示人白细胞ＤＲ抗原（ＨＬＡ－ＤＲ）和第Ⅷ因子相关抗原（ＦⅧＲＡｇ）的表达规律，研究血管内皮细胞的生物学行为。方法应用ＨＬＡ－ＤＲ和ＦⅧＲＡｇ的抗血清，对３９例淋巴瘤中血管内皮细胞的功能异质性进行免疫组织化学及其形态定量研究，同时取新生儿、成人淋巴结及反应性增生淋巴结做对照。结果新生儿、成人淋巴结及反应性增生淋巴结内各类血管内皮细胞对ＨＬＡ－ＤＲ、ＦⅧＲＡｇ均呈阳性，其中高内皮后微静脉（ＨＥＶ）呈强阳性；Ｈｏｄｇｋｉｎ病和Ｔ细胞淋巴瘤中血管多数是ＨＥＶ样血管，内皮细胞均表达ＨＬＡ－ＤＲ及ＦⅧＲＡｇ；Ｂ细胞淋巴瘤中血管多数是毛细血管样血管，内皮细胞很少表达或不表达ＨＬＡ－ＤＲ，只表达ＦⅧＲＡｇ。结论说明Ｈｏｄｇｋｉｎ病和Ｔ细胞淋巴瘤中血管内皮细胞既参与免疫调节过程，又参与凝血过程；而Ｂ细胞淋巴瘤内血管内皮细胞只参与凝血过程，不参与免疫调节过程。Objective To determine the heterogenicity in the expression of class Ⅱ major histocompatible antigen (HLA DR), factor Ⅷ related antigen (FⅧRAg) and the biological behavior of vessel endothelial cells in malignant lymphoma. Methods Standard immunohistochemical method using antisereum of HLA DR and FⅧRAg were applied to detect hetereogeneous expression of endothlial cells in 39 cases of malignant lymphomas using neonatal lymph nodes, normal adult lymph nodes and lymph node hyperplasia as the controls. Results Both HLA DR and FⅧRAg reactivity was found in blood vessels in neonate lymph nodes, normal adult lymph nodes and lymph node hyperplasia. Most vessels in Hodgkin′s disease and T cell lymphoma had high endothelial post capillary venules (HEV), which expressed both FⅧRAg and HLA DR. In B cell lymphoma, most blood vessels are capillary like microvessels, which expressed little or no HLA DR, only expressing FⅧRAg. Conclusions The above results indicate that the endothelial cell of blood vessels in Hodgkin′s disease and T cell lymphoma not only participate in immunoregulation but also in blood coagulation, whereas, those in B cell lymphoma only participate in blood coagulation.

关 键 词：淋巴瘤 血管内皮 α白细胞 DR抗原 Ⅷ因子 

分 类 号：R733.402[医药卫生—肿瘤]