胃癌组织TSER多态性与5-Fu体外药敏的关系  

Correlation between TSER Polymorphism and Sensitivity of Gastric Cancer Tissue to 5-Fluorouracil

作　　者：贺文兴[1] 邓觐云[1] 孙正魁[1] 王艳华[1] 陈文学[1] 刘弘晟[2] 

机构地区：[1]江西省肿瘤医院,南昌330029 [2]南昌大学研究生院医学部

出　　处：《肿瘤防治研究》2009年第2期126-129,共4页Cancer Research on Prevention and Treatment

基　　金：江西省卫生厅科技计划基金资助项目(20071119)

摘　　要：目的探讨胃癌组织胸苷酸合成酶增强子区(TSER)多态性与5-Fu体外药敏的关系。方法对45例胃癌患者新鲜标本进行体外肿瘤细胞原代培养,用ATP-TCA法检测肿瘤细胞对5-Fu的敏感性并用PCR法检测肿瘤的TSER多态性,分析TSER多态性分布频率与体外药敏有效率的关系。结果TSER多态性三种基因型频率分布:2R/2R、2R/3R、3R/3R基因组分别为6.7%(3/45)、31.1%(14/45)和62.2%(28/45)。5-Fu总有效率为33.3%(14/42)。3R/3R基因组26例,有效5例,无效21例,有效率19.2%;(2R/2R、2R/3R)基因组16例,有效9例,无效7例,有效率56.3%。TSER基因型组之间对5-Fu敏感性存在显著差异性(P<0.05)。结论TSER多态性对5-Fu化疗敏感性可能有关,基因型检测可能有助于预测胃癌组织对5-Fu化疗敏感性。Objective To investigate correlation between thymidylate synthase enhancer region （TSER） polymorphism and the antitumor activities of 5-fluorouracil （5-Fu） determined by ATP sensitivity assay in vitro. Methods The carcinoma cells isolated from fresh gastric tissue in 45 patients were cultured in vitro. The sensitivity of these cells to 5-Fu were tested using ATP TCA method. TSER genotypes were detected by PCR and the correlation between different of TSER genotypes and its chemosensitivity to 5-Fu was analyzed in this study. Results In all cases, the frequency distribution of TSER 2R/2R,2R/3R and 3R/3R genotypes were 6. 7 % （3/45）,31. 1% （14/45） and 62. 2% （28/45）, respectively. The overall effective rate of 3R/3R group to 5-Fu was 33.3%（14/42）. In 2R/2R and 2R/3R genotype group its effective rate to 5 Fu was 56. 3% （9/16）, which was significantly higher than that of 3R/3R genotype group（19. 2%,P〈0.05）. Conclusions The results in the present study suggest that the polymorphism of TSER was associated with the antitumor activities of 5-fluorouracil, indicating that analysis of TSER polymorphisms might be useful to direct 5 fluorourcil based antitumor chemotherapy.

关 键 词：胃癌 胸苷酸合成酶增强子区多态性 5-氟尿嘧啶 体外药敏试验 

分 类 号：R735.2[医药卫生—肿瘤]