乳腺癌差异表达的MicroRNA的筛选研究  

Identification of Differentially Expressed MicroRNAs in Breast Cancer

作　　者：黄秀芳[1,2] 邵建永[1] 颜黎栩[1] 李晓霞[1] 杜紫明[1] 邓玲[1] 梁小曼[1] 

机构地区：[1]华南肿瘤学国家重点实验室∥中山大学肿瘤防治中心病理科,广东广州510060 [2]江门市中心医院病理科,广东江门529030

出　　处：《中山大学学报（医学科学版）》2009年第1期69-73,77,共6页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家重点基础研究规划项目(“973”计划,2004CB518708);广东省科技计划项目(2003A3080202);广州市科技计划项目(2003I-E0341)

摘　　要：【目的】应用miRNA芯片技术筛选乳腺癌及癌旁组织差异表达的miRNA,发现与乳腺癌相关的miRNA,为进一步阐明其在乳腺癌发病机制中的作用打下基础。【方法】收集8例新鲜的乳腺癌及其癌旁组织,抽提总RNA并分离出小RNA进行Cy3荧光标记,将标记的小RNA在miRNA芯片上进行杂交反应,应用实时定量RT-PCR方法验证miRNAs芯片结果的可靠性。【结果】对芯片数据进行SAM分析,结果筛选获得16个乳腺癌相关miRNAs,相对于癌旁组织,在乳腺癌组织中9个miRNAs表达上调,7个miRNAs表达下调。其中显著上调的有miR-21,miR-365,显著下调的有miR-497,miR-31。【结论】筛选得到乳腺癌miRNA差异表达谱,其可能与乳腺癌的发生发展有关。[Objective] To screen and identify the miRNA differential expression profile in breast cancer （BRCA） by the miRNA array technique to make further study on the function of miRNA in pathogenesis of BRCA. [Methods] Total RNA was extracted from 8 breast tumor samples and normal adjacent tissues. Small miRNA was isolated from total RNA, labeled with Cy3 and hybridized on miRNA array. Real time quantitative PCR was applied to verify the reliability of miRNA array results. [Results] By significance analysis of microarrays （SAM） based on microarray screening, 16 BRCA related miRNAs were obtained. In BRCA, 9 up-regulated and 7 down-regulated miRNAs were observed. The most significant up-regulated miRNAs were miR-21 and miR-365, while miR-497 and miR-31 were significantly down-regulated. [Conclusion] miRNA differential expression profile of human BRCA was obtained, which may be related to the pathogenesis and development of BRCA.

关 键 词：MIRNA 乳腺癌 MIRNA芯片 实时定量RT-PCR 

分 类 号：R737.9[医药卫生—肿瘤]