HCV基因转染的肝癌细胞体内外生长的实验研究  

In vivo and in vitro study of cell growth of HCV genome transfected hepatocellular carcinoma

作　　者：曹文俊[1] 吴华成[2] 徐锋[3] 叶挺军[1] 许海敏[2] 樊绮诗[1] 

机构地区：[1]上海交通大学医学院瑞金医院检验科,上海200025 [2]上海交通大学医学院病理科,上海200025 [3]上海交通大学医学院仁济医院嘉定分院检验科,上海201800

出　　处：《上海交通大学学报（医学版）》2009年第2期158-161,共4页Journal of Shanghai Jiao tong University:Medical Science

基　　金：上海交通大学医学院百人计划(2004年)~~

摘　　要：目的研究丙型病毒性肝炎病毒(HCV)感染对宿主肝细胞体外生长速度和体内肝癌生长特性的意义。方法体外培养HCV基因转染的肝癌细胞(HepG2-HCV)和对照细胞(HepG2-vector),以MTT比色法测定两种细胞的生长速度。19只裸鼠分为三组:MEM培养液组(n=6)、HepG2-HCV组(n=7)和HepG2-vector组(n=6),分别于皮下注射无血清MEM培养液、HepG2-HCV、HepG2-vector细胞各0.2 mL,细胞密度为5×107/mL,观察皮下肿瘤的形成情况,并对小鼠肿瘤和肝、脾、肾、肺、心等脏器作病理学分析。结果HepG2-HCV的生长速度较对照细胞明显加快,在无血清培养条件下其生长优势更显著。体内成瘤实验结果显示,HepG2-HCV和HepG2-vector两种细胞使实验鼠形成皮下肿瘤的比例分别为71.43%和83.33%,两组间差异无统计学意义(P>0.05)。病理学分析表明,HepG2-HCV组形成的5例实验肿瘤生长良好,片状坏死的发生率为0;HepG2-vector组5例肿瘤组织中均存在片状坏死,发生率为100%,两组间差异有统计学意义(P<0.05)。两组肝、脾、肾、肺、心等脏器的病理学分析均未见明显异常。结论HCV促进肝癌细胞加速生长,并使组织中肿瘤细胞处于良好生长状态。Objective To study the impact of hepatitis C virus （HCV） infection on host hepatocyte growth in vitro and in vivo. Methods HCV genome transfected hepatocellular carcinoma （HepG2-HCV） cells and control cells （HepG2-vector cells） were cultured in vitro, and cell growth velocity was detected using MTT method. Nineteen nude mice were divided into MEM culture fluid group （n =6）, HepG2-HCV group （n = 7） and HepG2-vector group （n =6）, and were subcutaneously injected with serum free MEM culture fluid （0.2 mL） , HepG2-HCV cells （0.2 mL） and HepG2-vector cells （0.2 mL）, respectively, with the cell density of 5 ~ 107/mL. The formation of subcutaneous tumors was observed, and pathological observation was conducted for tumor, liver, kidney, heart, lung and spleen. Results The growth of HepG2-HCV cells was much faster than that of control cells, and the growth advantage was more significant under culture with serum free medium. It was revealed by in vivo tumor formation test that there was no significant difference in the rate of subcutaneous tumor formation between HepG2-HCV cells and HepG2-vector cells （71.43% vs 83.33% , P 〉 0.05）. It was revealed by pathological observation that the five formed tumors in HepG2-HCV group grew well with no incidence of patchy necrosis, while patchy necrosis was found in tissues of all the formed tumors in HepG2-vector group. There were no abnormal pathological findings in liver, spleen, kidney, lung and heart. Conclusion HCV can stimulate the growth of hepatocellular carcinoma and ensure a ia,ou：able growth environment for the tumor cells.

关 键 词：丙型肝炎病毒 肝细胞肝癌 细胞生长 

分 类 号：R735.7[医药卫生—肿瘤]