重组人促红细胞生成素对急性高眼压兔眼视网膜缺氧诱导因子1α表达的影响  

Effects of recombinant human erythropoietin on hypoxia inducible factor-1α expression in the retina of rabbits with acute high intraocular pressure

作　　者：王建明[1] 宋艳萍[2] 孙乃学[1] 惠娜[1] 赵世平[1] 胡凯[2] 

机构地区：[1]西安交通大学医学院第二附属医院眼科,陕西西安710004 [2]西安市中心医院血液科,陕西西安710003

出　　处：《南方医科大学学报》2009年第2期271-273,277,共4页Journal of Southern Medical University

基　　金：陕西省科技攻关资助项目(2008K14-07);西安市社发医药卫生研究资助项目(SF08004-2)

摘　　要：目的观察重组人促红细胞生成素(rhEPO)对急性高眼压兔眼视网膜缺氧诱导因子-1α(HIF-1α)表达的影响,探讨国产rhEPO保护视网膜神经功能的机制。方法在设立正常对照、模型对照的情况下,日本大耳白兔皮下注射国产rhEPO,分别于缺血再灌注(生理盐水前房灌注法造成急性高眼压)后第1、3、7、14天免疫组化法观察视网膜HIF-1α基因表达。结果正常兔眼视网膜无HIF-1α表达。模型组兔眼视网膜HIF-1α表达阳性细胞数较正常对照组明显增加(P<0.01),EPO组兔眼视网膜HIF-1α表达阳性细胞数变化与模型组相似,但较模型组显著减少(P<0.01)。结论国产rhEPO能够下调视网膜HIF-1α的表达。下调视网膜HIF-1α的表达可能是rhEPO对视网膜缺血再灌注损伤的保护机制之一。Objective To observe the effect of recombinant human erythropoietin （rhEPO） on the expression of hypoxia inducible factor-1α（HIF-1α） in the retina of rabbits with acute high intraocular pressure and investigate the mechanism of rhEPO in protecting the retina from ischemia-reperfusion injury. Methods Acute high intraocular pressure was induced in the rabbits by perfusion of normal saline into the anterior chamber, and rhEPO was injected subcutaneously. The changes in HIF-1α protein expression in the retina was observed by immunohistochemistry on days 1, 3, 7, and 14 after retinal ischemiareperfusion. Results HIF-1α expression was not observed in the retina of the normal control rats, but intense HIF-1α expression was found in the model group （P〈0.01）. In rabbits with rhEPO injection and those in the model group, the patterns of HIF-1α expression alterations were similar, but the HIF-1α-positive cells in the retina were significantly fewer in rhEPO group （P〈0.05）. Conclusion rhEPO can down-regulate HIF-1α expression in the retina of rabbits with acute high intraocular pressure, which may be one of the mechanisms that rhEPO protects the retina from ischemia-reperfusion injury.

关 键 词：重组人促红细胞生成素 视网膜 缺氧诱导因子-1Α 高眼压 

分 类 号：R774.1[医药卫生—眼科]