阿托伐他汀对人胆管癌QBC939细胞系增殖及侵袭的影响  

Effects of atorvastatin on the proliferation and invasiveness of human cholangiocarcinoma QBC939 Cells

作　　者：陈有挺[1] 郑龙志[2] 何庆良[1] 石铮[1] 林永堃[1] 

机构地区：[1]福建医科大学附属第一医院普通外科,福建福州350005 [2]莆田学院附属医院普通外科,福建莆田351100

出　　处：《中国普通外科杂志》2009年第2期136-139,共4页China Journal of General Surgery

基　　金：福建省卫生厅青年科研课题资助(2007-1-12)

摘　　要：目的探讨阿托伐他汀(ATV)对人胆管癌QBC939细胞系增殖、侵袭的影响及其可能作用机制方法应用细胞培养技术培养QBC939细胞,经不同浓度的阿托伐他汀处理后,以MTT法检测阿托伐他汀对QBC939细胞的杀伤抑制率,以Matrigel侵袭实验、迁移实验和半定量RT-PCR检测阿托伐他汀对QBC939细胞的侵袭、运动能力和对细胞内RhoC,MMP-9,p27 mRNA表达的影响情况。结果阿托伐他汀可明显抑制QBC939细胞的生长及增殖,且其抑制作用呈剂量-时间效应关系:IC50为25μmol/L,最强抑制作用时间为48h。Matrigel侵袭实验及迁移实验显示,经10μmol/L,25μmol/L,50μmol/L药物干预48h后的QBC939细胞,随着浓度的增加,其体外侵袭、运动能力明显减弱(P<0.05);半定量RT-PCR测定显示,阿托伐他汀作用48h后,QBC939细胞中p27表达含量明显增高、MMP-9的表达降低,而RhoC的表达改变并不明显。结论阿托伐他汀可抑制人胆管癌QBC939细胞的生长增殖,并减弱其体外侵袭、运动能力。Objective To investigate the effects of atorvastatin on the proliferation and invasion of human cholangiocarcinoma QBC939 cells, and explore the possible mechanisms. Methods The human eholangiocarcinoma QBC939 cell line was incubated with different concentrations of atorvastatin for 48h. MTT assay for cell growth and viability, Boyden chamber for invasion and motility assays, and semiquantitative RT-PCR to determine mRNA of p27, MMP-9 and RhoC. Results The results showed that atorvastatin inhibited growth and proliferation of QBC939 cells, with a time- and dose-dependent inhibition, and at an IC50 value of 25 umol/L, inhibition was most marked at 48 h. Al50, atorvastatin-treated （10 μmol/L, 25μmol/L,50μmol/L for 48 h） cells were less motile and invasive. Semiquantitative RT-PCR analysis showed that atorvastatin treatment reduced MMP-9 expression and upregulated the cyclin-dependent kinasc inhibitor p27 in QBC939 cells, but RhoC was not significantly altered between the experimental groups.Conclusions Atorvastatin can inhibit proliferation and invasion of human cholangiocarcinoma QBC939 cells.

关 键 词：胆管肿瘤 阿托伐他汀 人胆管癌QBC939细胞 细胞增殖 肿瘤侵润 

分 类 号：R735.8[医药卫生—肿瘤]