SELDI—TOF—MS技术筛选Paget病患者血清特异性蛋白质的研究  

Screening the serum proteomic patterns of Paget disease with SELDI-TOF-MS

作　　者：陈晋广[1] 杨志波[2] 朱明芳[2] 任小丽[1] 

机构地区：[1]浙江台州市立医院皮肤科,浙江台州318000 [2]湖南中医药大学附二院皮肤科

出　　处：《中国医师杂志》2009年第2期167-169,共3页Journal of Chinese Physician

基　　金：浙江省医药卫生科技计划（20078234）

摘　　要：目的探讨表面增强激光解析电离飞行时间质谱（SELDI—TOF—MS）技术在Paget病的诊断价值。方法用弱阳离子蛋白芯片（WCx2）及表面加强激光解析电离飞行时间质谱技术（SELDI—TOF—MS）检测15例健康者、15例慢性湿疹和20例Paget病患者血清中蛋白的相对含量。结果Paget病患者与健康人相比，质荷比为3868Da和8876Da的2个蛋白峰差异有统计学意义（P〈0．01）；与慢性湿疹相比，质荷比为2911、3868、5097Da的3个蛋白质峰差异有统计学意义（P〈0．05）。结论SELDI—TOS—MS的特异性肿瘤标记物对Paget病的早期诊断和鉴别慢性湿疹具有诊断价值，无创安全。Objective To evaluate the diagnostic value of surface enhanced laser desorption/ionization time of flight mass spectrometry（SELDI-TOF-MS） for Paget disease. Methods The relative contents of serum proteins of 15 healthy people, 15 patients with chronic eczema and 20 patients with Paget were detected by Weak cation exchanger protein chip （WCx2） and SELDI-TOF-MS. Results The contents of two proteins （3868Da,8876Da） had significant difference in healthy people and Paget disease patients （ P 〈 0. 01 ）. 2911Da, 3868Da and 5097Da protein peaks had significant difference in chronic eczema and Paget disease（ P 〈0. 05）. Conclusion It shows great potentiality for early diagnosis and screening the tumor biomarkers of Paget disease with SELDI-TOF-MS.

关 键 词：光谱分析 质量 蛋白质阵列分析 佩吉特病 乳腺外/诊断 诊断 鉴别 

分 类 号：R73[医药卫生—肿瘤]