组织工程化类金刚石膜复合材料与人血管内皮细胞的相容性研究(英文)  

Biocompatibility between tissue-engineering diamond-like carbon film and human vascular endothelial cells

作　　者：程光存[1] 严中亚[1] 罗乐[2] 方晓东[3] 沙自明[1] 

机构地区：[1]安徽医科大学附属省立医院心脏外科,安徽省心血管病研究所,安徽省合肥市230001 [2]合肥工业大学,安徽省合肥市230009 [3]中科院安徽光学精密机械研究所,安徽省合肥市230031

出　　处：《中国组织工程研究与临床康复》2009年第3期566-570,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：Anhui Talents Training and Scientific Research Foundation,No.[2005]58~~

摘　　要：目的:验证纳米相类金刚石薄膜复合材料与人血管内皮细胞相容性,为组织工程化机械瓣膜材料的构建提供依据。方法:利用脉冲激光沉积法在人工心脏机械瓣膜上沉积纳米相类金刚石薄膜,将人脐静脉血管内皮细胞与纳米相类金刚石薄膜复合材料体外复合培养。倒置显微镜及扫描电镜观察细胞在材料表面的生长、附着情况;MTT法检测细胞在材料上增殖情况;同时分别测定人脐静脉血管内皮细胞在类金刚石薄膜材料和空白对照组中一氧化氮及前列环素分泌水平,以评价其活性。结果:人脐静脉血管内皮细胞能在纳米相类金刚石薄膜复合材料上良好地黏附、增殖、生长。人脐静脉血管内皮细胞分泌一氧化氮和前列环素水平在类金刚石薄膜材料和空白对照组没有显著性差异(P>0.05),说明纳米相类金刚石薄膜材料对人脐静脉血管内皮细胞的活性没有影响。结论:纳米相类金刚石薄膜复合材料具有良好的细胞相容性,有可能作为组织工程化机械瓣膜材料。OBJECTIVE： To verify the biocompatibility between diamond-like carbon （DLC） film and human vascular endothelial cells and to provide evidences for construction of artificial mechanical valve prosthesis, METHODS： Nanophase DLC film was deposited using pulse laser deposition, while vascular endothelial cells derived from human umbilical vein was cultured with nanophase DLC film in vitro. Cell growth and adhesion were observed under inverted microscope, and cell proliferation was measured with MTT method. In addition, levels of nitric oxide （NO） and prostacyclin （PGI2） were measured in the DLC group and blank control group in order to evaluate their activities. RESULTS： Adhesion, proliferation, and growth of vascular endothelial cells derived from human umbilical vein were great on the surface of nanophase DLC film. There were no significant differences in the levels of NO and PGI2 between DLC group and blank control group （P 〉 0.05）, showing that nanophase DLC film had no effect on activity of vascular endothelial cells derived from human umbilical vein. CONCLUSION： Nanophase DLC film has a good biocompatibility, and it can become an ideal material of tissue-engineering artificial mechanical valve prosthesis.

关 键 词：脉冲激光沉积 类金刚石膜 组织工程 人血管内皮细胞 细胞培养 细胞相容性 

分 类 号：R318[医药卫生—生物医学工程]