磷脂酰甘油分子种与杨树抗寒性关系的研究  

STUDIES ON COMPOSITION OF MOLECULAR SPECIES OF PHOSPHATIDYLGLYCEROL IN RELATION TO COLD RESISTANCE OF POPLAR 

作　　者：沈漫[1] 黄敏仁[1] 王明庥[1] 胡兹苓[1] 包宏[1] 

机构地区：[1]南京林业大学林木遗传与基因工程林业部重点实验室

出　　处：《Acta Botanica Sinica》1998年第4期349-355,共7页Acta Botanica Sinica（植物学报：英文版）

基　　金："九五"国家科委攻关;国家自然科学基金

摘　　要：用高效液相色谱（ＨＰＬＣ）方法分析了杨树叶片磷脂酰甘油（ＰＧ）的分子种组成，用酶解和气相色谱（ＧＣ）方法分析了ＰＧ脂肪酸定位，比较了抗寒性不同的杨树（Ｐｏｐｕｌｕｓｄｅｌｔｏｉｄｅｓｃｖ．ＬｕｘＩ６９／５５和Ｐｏｅｕｒａｍｅｒｉｃａｎａｃｖ．Ｉ４５／５１）叶片ＰＧ分子种。结果表明，杨树叶片ＰＧ主要含有以下的分子种脂肪酸组成（ｓｎ１和ｓｎ２）∶１８∶３／１８∶２（１８∶２／１８∶３），１８∶３／１６∶１（３ｔ）；１８∶３／１６∶０；１８∶２／１６∶１（３ｔ）；１６∶０／１８∶２，１８∶２／１６∶０；１８∶１／１６∶１（３ｔ）；１６∶０／１６∶１（３ｔ）；１８∶１／１８∶１，１６∶０／１８∶１（１８∶１／１６∶０）；１６∶０／１６∶０。１６∶１（３ｔ）位于ｓｎ２位，１６∶０在ｓｎ１位和ｓｎ２位均有发现，Ｃ１８脂肪酸主要在ｓｎ１位，两种杨树叶ＰＧ的不饱和分子种都在７０％以上。抗寒性强的欧美杨Ⅰ４５的不饱和分子种／饱和分子种比率为３．１０，大于不抗寒的美洲黑杨Ⅰ６９的比率，杨树叶ＰＧ的饱和分子种含量之和∑［（１６∶０／１６∶０＋１６∶０／１６∶１（３ｔ）］与抗寒性密切相关，Ⅰ６９杨叶片ＰＧ的∑?The composition of molecular species and the positional distribution in fatty acids of phosphatidylglycerol (PG) isolated from poplar (Populus deltoides cv.Lux 1 69/55 and Poeuramericana cv.I-45/51) leaves were analyzed by high performance liquid chromatography (HPLC), enzym hydrolysis and gas phase chromatography (GC), and the different cold resistant poplars were compared with respect to the compositions of molecular species of PG isolated from their leaves. The results showed that the fatty acid compositions (sn 1/sn 2) of the major molecular species in PGs from poplar leaves were as follows: 18∶3/18∶2(18∶2/18∶3),18∶3/16∶1(3t);18∶3/16∶0;18∶2/16∶1(3t);16∶0/18∶2,18∶2/16∶0;18∶1/16∶1(3t);16∶0/16∶1(3t);18∶1/18∶1,16∶0/18∶1(18∶1/16∶0);16∶0/16∶0。The positional distribution of fatty acids in PG from poplar leaves was found that 16∶1(3t) was exclusively occupied the sn 2 position, whereas 16∶0 was present in both the sn 1 position and the sn 2 position. The C 18 acids were principally localized at the sn 2 position. The relative contents of the unsaturated molecular species of leaf PGs were more than 70% in both cold resistant poplar and cold sensitive poplar. The ratio of the unsaturated/ saturated molecular species of PG isolated from the cold resistant Ⅰ 45 poplar was 3.10, which was higher than that of the PG from the cold sensitive cottonwood, which was 2.38. The sum of the relative contents of the disaturated molecular species of the PG from poplar leaves was closely associated with the cold resistance of plants. The ∑[16∶0/16∶0+16∶0/16∶1(3t)] of the PG from cottonwood was higher than that of the PG from cold resistant Ⅰ 45 poplar. The differences in the compositions of molecular species and the phase transition temperatures of PGs between cold resistant and cold sensitive plants were discussed in terms of the pathways and the activities of selective acyltransferases involved in the PG biosynthesis in chloroplast.

关 键 词：杨树 磷脂酰甘油 分子种 脂肪酸 抗寒性 

分 类 号：Q948.112.2[生物学—植物学] S792.111[农业科学—林木遗传育种]