肝癌发生过程中核因子-κB和Bcl-2动态表达及意义  

Dynamic Expression of Apoptosis Inhibitory Protein NF-κB and Bcl-2 during the Course of Hepatocyte Carcinogenesis in Rats

作　　者：李立人[1] 姚登福[1] 顾青青[1] 宗蕾[1] 吴玮[1] 吴信华[1] 邱历伟[1] 

机构地区：[1]南通大学附属医院消化内科,江苏南通226001

出　　处：《中国临床医学》2009年第1期67-69,共3页Chinese Journal of Clinical Medicine

基　　金：江苏省"六大人才高峰"项目资助(NO:2006-B-063)

摘　　要：目的:探讨肝癌形成过程中核因子-κB(NF-κB)及Bcl-2的动态表达与临床意义。方法:以2-乙酰氨基芴(2-FAA)喂雄性SD大鼠制备肝癌模型,病理分析肝细胞的形态学变化,在癌变不同时期,分别以免疫组织化学法观察NF-κB和Bcl-2变化,并定量观察核蛋白、NF-κB和Bcl-2动态表达。结果:病理检查示鼠在喂2-FAA后,正常肝细胞在诱癌早期发生颗粒样变性,中期肝组织出现不典型增生,后期有大量癌巢结节,均为高分化肝细胞癌。在正常肝细胞中仅见较低水平的NF-κB和Bcl-2表达。诱癌后,随着肝细胞形态的变化,肝组织中NF-κB和Bcl-2表达依次增强,均显著高于正常肝组织(P<0.01),且两者的表达高度相关(r=0.73,P<0.01)。结论:癌变过程中NF-κB信号转导途径激活和Bcl-2呈过表达状态与肝癌的发生、发展密切相关。Objective：To investigate the kinetic expression and altered mechanism of hepatic apoptosis inhibitory protein nuclear factor Kappa B （NF-κB） and Bcl-2 during the course of hepatoeyte carcinogenesis. Methods： Rat hepatoma models were induced with 2-acetamidoflurene （2-FAA） on male SD rats. Morphological changes were observed by pathological examinations （HE staining）. Dynamic alterations of nuclear proteins, NF-κB and Bcl-2 at different stages of canceration were quantitatively analyzed by immuno histochemsitry or ELISA, respectively. Results： The rat hepatocytes showed vacuole-like denaturation at the early stages, then hyperplastic nodal appearance at middle stage, and finally progression to tubercles of cancerous nest with highly differentiated hepatocyte cancers during development of rat HCC by Histological examinations. An increasing tendency of liver NF-κB and Bcl-2 expression was found from normal liver to precancerous to cancerous tissues during rat hepatoma de velopment and comparison with lower expression in normal controls （P〈0. 01）, and closely relationship was found between them （r = 0.73, P〈0. 01 ）. Conclusion：The reactivation of NF-κ3 signal transduction pathway and overpression of hepatic Bcl- 2 are closely associated with occurrence and development of hepatocyte eanceration.

关 键 词：肝细胞肝癌 核因子-ΚB BCL-2 早期表达 大鼠 

分 类 号：R735.7[医药卫生—肿瘤]