长期吸烟对肺癌癌旁及周围肺组织结构和P53、VEGF蛋白表达的影响  

Effect of Long-term Smoking on Structure and Protein Expression of P53 and VEGF in Pericancer and Surrounding Lung Tissue.

作　　者：李若葆[1] 黄琰[2] 王金平[1] 薄其付[1] 李洪先[1] 

机构地区：[1]山东省潍坊医学院人体解剖学教研室,261053 [2]山东省潍坊医学院呼吸内科,261053

出　　处：《医学研究杂志》2009年第3期24-27,共4页Journal of Medical Research

基　　金：山东省自然科学基金课题(Y2007C044)

摘　　要：目的本研究旨在探讨吸烟对肺癌患者癌组织、癌旁组织以及周边组织P53和VEGF的表达,为吸烟者肺癌的早期诊断提供理论依据。方法选择肺癌患者126病例,其中吸烟组96例,非吸烟组30例,所有病例临床资料完整,吸烟史明确,患者术前均未接受化疗、放疗或其他针对肿瘤的治疗。光镜下观察支气管黏膜上皮、肺癌组织、癌旁组织和周围组织等结构变化情况,免疫组织化学检测吸烟组及非吸烟组肺癌组织、癌旁组织及周边组织P53和VEGF蛋白的表达情况。所得数据采用SAS软件作统计学处理分析,比较各组间差异程度。结果吸烟组肺泡腔不规则扩大,肺泡壁变薄或断裂,呼吸性细支气管呈囊状扩张,细支气管壁明显增厚,严重者支气管黏膜上皮排列紊乱、脱落;细胞表面的分泌物及杯状细胞和腔内黏液栓均较非吸烟组多。P53蛋白表达于细胞核,VEGF表达于细胞质以及新生血管内皮细胞,阳性均呈棕黄色颗粒状。癌组织P53、VEGF阳性表达均高于癌旁组织及周边组织(P<0.05);吸烟组癌旁组织和周边组织P53、VEGF阳性表达均高于非吸烟组(P<0.05)。P53、VEGF在吸烟组和非吸烟组肺癌组织中的表达均呈正相关,提示P53基因突变可能上调VEGF表达,突变型P53基因和VEGF在肺癌血管形成中可能具有协同作用。结论吸烟可引起肺组织损害,并使肺组织P53和VEGF蛋白表达增强,P53和VEGF表达与肺组织损害呈正相关。Objective To investigate the effect of expressions of P53 and VEGF in smoking lung cancer and surrounding lung tissue, and to offer us with theorial evidence of early diagnosing smoking - related lung carcinoma clinically. Methods 126 lung cancer patients were recruited, including 96 long - term smoking and 30 nonsmoking patients. All clinical data was integrity and the patients had clear smoking history. None of patients underwent chemotherapy, radiotherapy and other tumor treatment. The bronchial epithelium, cancer tissues, pericancer lung tissues, surrounding lung tissues were observed by light microscopy, and the expressions of P53 and VEGF of lung cancer tissues, pericancer lung tissues and surrounding lung tissues were detected by Immunohistochemical methed in smoking and non - smoking group. The experimental data was analyzed by SAS statistical software and the degree of difference between the groups was compared accordingly. Results There were different levels of expansion of alveolar wall in lung tissues of smokers, and alveolar wall became capsular to expand. Respiratory bronchioles were cystic expansion and small bronchial wall becomed thickening, with severe bronchial epithelial membrane ranking nuts - chaos and peeling. Goblet cells and the cell surface of the cavity mucous secretions and suppositories were more than those of non - smokers. The P53 protein was expressed in cell nucleus and VEGF expressed in the cytoplasm and endothelial cells of neovascularization. Both their positive behavior was granular brown. Either for smoking or for non - smoking lung cancer, the expressions of P53 and VEGF were higher in tumor tissues than that of in the perieancer and surrounding lung tissue （P 〈 0.05 ）. It was higher that of the expressions of P53 and VEGF in smoking pericancer and surrounding lung tissues than that of non - smoking （P 〈 0. 05 ）. Either in smoking or in non - smoking pationts, the expressions of P53 and VEGF was significantly increased. The expression of P53 positively was correl

关 键 词：吸烟 肺癌 P53 VEGF 免疫组织化学 

分 类 号：R735.34[医药卫生—肿瘤] R734.2[医药卫生—临床医学]