硒对乳腺癌细胞株p21^(WAF1/CIP1)启动子的调控作用  

Regulatingrole of p21^(WAF1/CIP1) promoter by selenium on breast cancer cell

作　　者：申社林[1] 李兵[1] 李朝争 李建广[1] 郭靠山[1] 

机构地区：[1]邢台医学高等专科学校基础部,河北邢台054000 [2]邢台市矿务局医院,河北邢台054000

出　　处：《现代肿瘤医学》2009年第3期436-438,共3页Journal of Modern Oncology

摘　　要：目的:研究硒对p21的转录调控及其调控位点。方法:通过向转染了重组质粒pGL3-p21p的乳腺癌细胞株MCF7先后加入不同的p21因子启动子的负调节因子和乳酸硒,对比分析荧光素酶表达活性,以确定硒对p21的转录调控及调控位点,并验证硒对癌细胞的生长的负调控作用。结果:perifosine、depsipeptide、apicidin、butyrate与硒共同诱导荧光素酶,酶活性表达无显著差异;而C-Myc与醋酸硒先后诱导酶活性表达差异显著。结论:硒对癌细胞具有诱导凋亡的作用,转录调节位点在p21启动子的sp1结合位点。Objective：To study the transcription and regulates site to the p21 by the selenium.Methods：Through adding different vice-regulative factor and selenium lactate to breast cancer cell line MCF7 which has transfected with pGL3-p21p,comparing the luciferase activity expression to determine the transcription regulating and regulate site to the p21 factor by selenium,and which has validated vice-regulative role to the growth of cancer cell.Results：Perifosine,depsipeptide,apicidin,butyrate and selenium induce luciferase altogether,and luciferase activity had no notable difference,P=0.336;While luciferase activity induced by C-Myc and selenium lactate had notable difference P=0.005.Conclusion：Selenium has the function of apoptosis induction to the cancer cell,and the transcription regulate site of selenium to p21 promoter located in sp1 binding sites.

关 键 词：p21~WAF1/CIP1 启动子 pGL3-p21p载体 荧光素酶 

分 类 号：R730.3[医药卫生—肿瘤]