重组蛋白的体外再折叠  

Refolding of Recombinant Proteins in Vitro

作　　者：龙建银[1] 王会信[1] 

机构地区：[1]军事医学科学院基础医学研究所生物化学室

出　　处：《生理科学进展》1998年第2期103-108,共6页Progress in Physiological Sciences

摘　　要：重组蛋白的再折叠是基因工程下游处理中非常重要的环节。本文在分析了蛋白体外折叠的机制后，指出了重组蛋白再折叠的一般策略，并综述了近年来的主要新方法，包括：分子伴侣介导的再折叠，去污剂协助的再折叠，反向微团中的蛋白再折叠。Refolding of recombinant proteins is an important event in the downstream process of genetic engineering. 　On the basis of the mechanism of protein folding in vitro, general strategy of recombinant protein refolding is postulated, and major developments in recent years on this field are reviewed, including: molecular chaperone mediated refolding, detergentassisted refolding, protein refolding in reverse micelle, addition of folding enhancer and methods for the formation of disulfide bond during protein refolding.

关 键 词：重组蛋白 再折叠 复性 

分 类 号：Q784[生物学—分子生物学] Q51